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Drosophila melanogaster

ABSTRACT: Transcriptomes of lineage-specific Drosophila neuroblasts profiled via genetic targeting and robotic sorting

PROVIDER: PRJNA290363 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2107300.fastq.gz	Fastqsanger.gz
	SRR2107310.fastq.gz	Fastqsanger.gz
	SRR2107320.fastq.gz	Fastqsanger.gz
	SRR2107301.fastq.gz	Fastqsanger.gz
	SRR2107311.fastq.gz	Fastqsanger.gz

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Transcriptomes of lineage-specific Drosophila neuroblasts profiled via genetic targeting and robotic sorting

Project description:A brain consists of numerous distinct neurons arising from a limited number of progenitors, called neuroblasts in Drosophila. Each neuroblast makes a specific neuronal lineage. To unravel the transcriptional networks that underlie the development of distinct neuroblast lineages, we marked and isolated lineage-specific neuroblasts for RNA sequencing. We labeled particular neuroblasts throughout neurogenesis by activating a conditional neuroblast driver in specific lineages using various intersection strategies. The targeted neuroblasts were efficiently recovered using a custom-built device for robotic single cell picking. Transcriptome analysis on the mushroom body, antennal lobe, and type II neuroblasts besides non-selective neuroblasts, neurons, and glia revealed a rich repertoire of transcription factors expressed among neuroblasts in diverse patterns. In addition to those likely pan-neuroblast transcription factors, there exist many transcription factors selectively enriched or repressed in certain neuroblasts. The unique combinations of transcription factors present in different neuroblasts may govern the diverse lineage-specific neuron fates

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