Project description:Objectives: determination of transcription start sites in Vibrio harveyi genome and discovery of new transcripts Methods: we performed differential seqencing of total RNA isolated from o.n. control Vibrio harveyi cultures. Sample treatment with Terminator EXonuclease (TEX) allowed differenciation of primary and secondary transcripts, helping in the definition of transcription start sites (TSS) Results: by data-mining RNA-seq data and performing some Northern Blot experiments we were able to detect new putative small-RNAs, along with these results, a more deep analisys of our RNA-seq data will give futher insight into genetic organization of Vibrio harveyi genome to help in its investigation
Project description:In this research, we used RNA-sequencing technology to detect genome-wide differentially expressed genes in spleen and gill of Vibrio harveyi -infected Takifugu rubripes.This high-throughput sequencing could help us to understand new mechanisms of action of V. harveyi induced aquaculture fish disease.
Project description:Vibrio harveyi, a significant pathogen in aquaculture, can lead to severe gut damage and metabolic disorders in fish. Verification via Transcriptome Sequencing of the gut damage caused by Vibrio harveyi in Hybrid Groupers and the Alleviating Effect of oligochitosan Feeding.
Project description:Vibrio harveyi, a significant pathogen in aquaculture, can lead to severe liver damage and metabolic disorders in fish. Verification via Transcriptome Sequencing of the Hepatic Damage Caused by Vibrio harveyi in Hybrid Groupers and the Alleviating Effect of Chitosan Oligosaccharide Feeding.
