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Bos taurus

ABSTRACT: The repressive regulatory role of H3K27me3 histone mark in bovine mastitis resistance to Staphylococcus aureus

PROVIDER: PRJNA290927 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2126920.fastq.gz	Fastqsanger.gz
	SRR2126921.fastq.gz	Fastqsanger.gz
	SRR2126922.fastq.gz	Fastqsanger.gz
	SRR2126917.fastq.gz	Fastqsanger.gz
	SRR2126918.fastq.gz	Fastqsanger.gz

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The repressive regulatory role of H3K27me3 histone mark in bovine mastitis resistance to Staphylococcus aureus

Project description:The regulatory effects of H3K27me3 on target genes expressions were analyzed by comparing S. aureus mastitis resistant and susceptible cows. The differentially expressed genes are mainly associated with immune and disease-related processes, which were negatively regulated by H3K27me3 modification on the up 2Kb regions relative to TSS in S. aureus mastitis cattle.

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Project description:Exploration of bovine milk exosomal microRNAs during Streptococcus uberis induced mastitis

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Global mapping of miRNA-target interactions in cattle (Bos taurus)

Project description:With regulatory roles in development, cell proliferation and disease, micro-RNA (miRNA) biology is of great importance and a potential key to novel RNA-based therapeutic regimens. Biochemically based sequencing approaches have provided robust means of uncovering miRNA binding landscapes on transcriptomes of various species. However, a current limitation to the therapeutic potential of miRNA biology in cattle is the lack of validated miRNAs targets. Here, we use cross-linking immunoprecipitation (CLIP) of the Argonaute (AGO) proteins and unambiguous miRNA-target identification through RNA chimeras to define a regulatory map of miRNA interactions in the cow (Bos taurus). The resulting interactome is the deepest reported to date for any species, demonstrating that comprehensive maps can be empirically obtained. We observe that bovine miRNA targeting principles are consistent with those observed in other mammals. Motif and structural analyses define expanded pairing rules with most interactions combining seed-based pairing with distinct, miRNA-specific patterns of auxiliary pairing. Further, miRNA-target chimeras had predictive value in evaluating true regulatory sites of the miR-17 family. Finally, we define miRNA-specific targeting for >5000 mRNAs and determine gene ontologies (GO) for these targets. This confirmed repression of genes important for embryonic development and cell cycle progress by the let-7 family, and repression of those involved in cell cycle arrest by the miR-17 family, but it also suggested a number of unappreciated miRNA functions. Our results provide a significant resource for transcriptomic understanding of bovine miRNA regulation, and demonstrate the power of experimental methods for establishing comprehensive interaction maps.

2017-07-11 | GSE90089 | GEO

Histone H3.3 lysine 9 and 27 control repressive chromatin states at cryptic cis-regulatory elements and bivalent promoters in mouse embryonic stem cells

Project description:Histone modifications are associated with distinct transcriptional states, but it is unclear whether they instruct gene expression. To investigate this, we mutated histone H3.3 K9 and K27 residues in mouse embryonic stem cells (mESCs). Here, we find that H3.3K9 is essential for controlling specific distal intergenic regions and for proper H3K27me3 deposition at promoters. The H3.3K9A mutation resulted in decreased H3K9me3 at regions encompassing endogenous retroviruses and induced a gain of H3K27ac and nascent transcription. These changes in the chromatin environment unleashed cryptic enhancers, resulting in the activation of distinctive transcriptional programs and culminating in protein expression normally restricted to specialized immune cell types. The H3.3K27A mutant disrupted deposition and spreading of the repressive H3K27me3 mark, particularly impacting bivalent genes with higher basal level of H3.3 at promoters. Therefore, H3.3K9 and K27 crucially orchestrate repressive chromatin states at cis-regulatory elements and bivalent promoters, respectively, and instruct proper transcription in mESCs.

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Project description:Small regulatory RNA in the bovine genome

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LncRNA-sequencing of bovine MAC-T cells

Project description:Escherichia coli and Staphylococcus aureus are two common pathogenic microorganisms that cause mastitis in dairy cows. They can cause clinical mastitis and subclinical mastitis. In recent studies, lncRNAs have been found to play an important role in the immune responses triggered by microbial inducers. However, the actions of lncRNAs in bovine mastitis remain unclear. The purpose of this study was to explore the lncRNA profile on mastitis.

2021-08-06 | GSE181464 | GEO

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Project description:Expression data from healthy (control) and Staph aureus mastitis cows' milk somatic cells (MSCs)

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Bos taurus

Project description:The effect of energy balance on bovine mammary tissue gene expression during Streptococcus uberis mastitis challenge

| PRJNA116573 | ENA

Bos taurus

Project description:A next generation sequencing approach to profiling the host miRNA response to a bovine mastitis pathogen

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