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Cardiocondyla obscurior

ABSTRACT: Transcriptomic signatures of mating and sham-mating in mature ant queens

PROVIDER: PRJNA293450 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2177530.fastq.gz	Fastqsanger.gz
	SRR2177540.fastq.gz	Fastqsanger.gz
	SRR2177531.fastq.gz	Fastqsanger.gz
	SRR2177541.fastq.gz	Fastqsanger.gz
	SRR2177532.fastq.gz	Fastqsanger.gz

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Project description:We performed a whole-genome transcriptomic analysis of pleometrotic queens infected by SINV-1, SINV-2 or co-infected with both viruses, to characterize patterns of gene expression associated with viral infection and identify genes responding differentially to the two viruses. We sampled fire ant queens in Gainesville, Florida, immediately after a mating flight. We arranged them in pairs based on having similar weights (range ±0.2 mg) to allow pleometrotic colony founding. We housed paired queens in nesting tubes in claustral conditions (no food or water) for one month. Thereafter, we collected queens in dry ice, we isolated total RNA from whole bodies and used it to quantify SINV-1 and SINV-2 viruses with real-time PCR. We selected 2 queens that were virus-free as controls, 3 SINV-1-infected, 4 SINV-2-infected and 11 co-infected by both viruses. These samples were processed for microarrays analysis.

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Effects of mating on gene expression profiles of mature ant queens

Project description:Will be added/updated once the manuscript is finalized. Cardiocondyla obscurior queens. Three treatments: virgin queens, queens mated by real males, queens sham-mated (by sterile males). Queens collected 1 week and 8 week after mating. seven loops for queens collected 1 week after mating; nine loops for queens collected 8 weeks after mating. Five direct comparisons (with dye-swaps... so 10 arrays) were done of between 1 week and 8 week samples of queens mated by real males. Each sample is RNA from two queens (from different colonies). Samples were hybridized against Solenopsis invicta microarrays (signal was detectable for most clones!)

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