Project description:Adult Sprague Dawley rats were treated i.g. with 50 mg/kg alpha-naphthylisothiocyanate (ANIT) or vehicle (corn oil). Hepatobiliary liver injury occurred at 24 h postdose in ANIT rats with repair at 120h. Livers were extracted from rats at 24h and 120 h post ANIT exposure. This study investigated differences in mRNA expression between the injury and repair phases in the context of ANIT exposure.
Project description:Adult Sprague Dawley rats were treated i.g. with 50 mg/kg alpha-naphthylisothiocyanate (ANIT) or vehicle (corn oil). Hepatobiliary liver injury occurred at 24 h postdose in ANIT rats with repair at 120h. Livers were extracted from rats at 24h and 120 h post ANIT exposure. This study investigated differences in mRNA expression between the injury and repair phases in the context of ANIT exposure. 8 week male Sprague Dawley rats were administered ANIT or vehicle for quantification of hepatobiliary injury via clinical chemistry biomarkers and pathology between 6 and 168 h postdosing. Rats sacrificed at 24 h and 120h postdosing coincided with peak injury and injury resolution, respectively.
Project description:A microarray analysis (transcriptome analysis) was conducted to examine estrogen-mediated gene expression in the arcuate nucleus (ARC) and paraventricular nucleus (PVN) of the hypothalamus, as well as the nucleus of the solitary tract (NTS) in female rats (Rattus norvegicus). All procedures were approved by the Oklahoma State University - Center for Health Sciences IACUC (protocol #2021-1278). Rats underwent ovariectomy, recovered, and were then treated with either 17β-estradiol (EB) or an oil vehicle (Oil). Following euthanasia, mRNA was extracted and submitted to Thermo Fisher Scientific for microarray analysis using the Clariom S Rat Pico Assay.
