Project description:We report the results of RNA sequencing of total RNA isolated from human pulmonary microvascular endothelial cells after transfection with human miR-887-3p or a negative control siRNA, both in triplicate.
Project description:In this study, we analzyed differences in the effect of hypoxia on the transcriptomic profile of cultured human pulmonary artery endothelial cells (HPAECs) vs. human brain microvascular endothelial cells (HBMVECs). To determine which hypoxia-regulated genes were dependent on hypoxia inducible factor 1-alpha (HIF-1alpha), some cells were transfected with an siRNA against HIF-1alpha prior to treatment with hypoxia. At the completion of the treatment protocol, mRNA was isolated using standard methodology and analyzed further by RNA-seq.
Project description:Transcriptional profiling of HPMECs comparing Control siRNA treated with HSP27 siRNA Two-condition experiment, control siRNA vs HSP27 siRNA ; 3 controlsiRNA transfected replicates , 3HSP27 siRNA replicates. Replicates were pooled and hybridized to a single array.
