Project description:Investigating the evolution of Escherichia coli in microgravity offers valuable insights into microbial adaptation to extreme environments. Here the effects of simulated microgravity (SµG) on gene expression of E. coli REL606, a strain evolved terrestrially for 35 years is explored. We evaluated the transcriptomic changes for glucose-limited and glucose-replete conditions over 24 hours which illustrate that SµG increased the expression of stress response and cell membrane-related genes, particularly under glucose-limited conditions. A machine learning model predicted that glucose-limited SµG impacts the cellular membrane, while glucose-replete SµG also inhibits protein synthesis at stationary phase. These findings highlight the transcriptomic and physiological adaptations of E. coli to short term microgravity, offering a foundation for future research into the long-term effects of space conditions on bacterial evolution.
Project description:The purpose of this study is to determine whether the presence of pathogenic Escherichia coli in colon is associated with psychiatric disorders.
Project description:Despite the characterization of many aetiologic genetic changes. The specific causative factors in the development of sporadic colorectal cancer remain unclear. This study was performed to detect the possible role of Enteropathogenic Escherichia coli (EPEC) in developing colorectal carcinoma.
Project description:we designed a CRISPR-based chromosome-doubling technique to construct an artificial diploid Escherichia coli cell. The stable diploid E. coli was confirmed by quantitative PCR and third-generation genome sequencing.
