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FOXO1 Regulates Attachment of the Implanting Blastocyst to the Endometrium at the Window of Receptivity

ABSTRACT: FOXO1 Regulates Attachment of the Implanting Blastocyst to the Endometrium at the Window of Receptivity

PROVIDER: PRJNA295289 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2339650.fastq.gz	Fastqsanger.gz
	SRR2339820.fastq.gz	Fastqsanger.gz
	SRR2339811.fastq.gz	Fastqsanger.gz
	SRR2339821.fastq.gz	Fastqsanger.gz
	SRR2339812.fastq.gz	Fastqsanger.gz

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FOXO1 Regulates Attachment of the Implanting Blastocyst to the Endometrium at the Window of Receptivity

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-07-03 | E-GEOD-72896 | biostudies-arrayexpress

FOXO1 Regulates Attachment of the Implanting Blastocyst to the Endometrium at the Window of Receptivity

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-10-15 | GSE72896 | GEO

FOXO1 Regulates Attachment of the Implanting Blastocyst to the Endometrium at the Window of Receptivity [RNA-seq]

Project description:We report the sequencing of the uterine transcriptome sequencing of control and conditional knock-out of the Forkhead box O1 at day 4.5 of murine pseudopregnancy RNA was isolated from day 4.5 Pseudopregnancy adult females. RNA from two females was pooled per sample. Three samples per genotype were sequenced for differential gene expression analysis.

2016-07-03 | E-GEOD-72895 | biostudies-arrayexpress

FOXO1 Regulates Attachment of the Implanting Blastocyst to the Endometrium at the Window of Receptivity [ChIP-seq]

Project description:We report the whole genome binding profile of the Forkhead Box O1 ChIP-seq in murine endometrium

2016-07-03 | E-GEOD-72892 | biostudies-arrayexpress

FOXO1 Regulates Attachment of the Implanting Blastocyst to the Endometrium at the Window of Receptivity [ChIP-seq]

Project description:We report the whole genome binding profile of the Forkhead Box O1

2018-12-31 | GSE72892 | GEO

Decreased epithelial Progesterone Receptor A at the window of receptivity is required for preparation of the endometrium for embryo attachment

Project description:The progesterone receptor A (PGRA) isoform is the dominantly expressed isoform within the mouse uterus and is indispensable for uterine function and fertility in the murine reproductive tract. During early pregnancy, the PGRA isoform is spatiotemporally expressed in the uterine epithelium. At days 2-3 of pregnancy, PGRA is upregulated within the epithelium and then decreases before day 4, the day of embryo implantation. This decrease of PGRA has been recognized as a marker to initiate the time in which the uterus is receptive to the embryo, yet this has never been functionally tested. We hypothesized the decrease of PGRA at the window of receptivity is required for successful embryo implantation. In order to test this hypothesis, we generated a PGRA conditional expression allele initiated by Cre recombinase present in the uterine epithelial compartment. The PGRA expressing mice exhibited infertility with the inability to undergo embryo implantation or decidualization. RNA microarray and ChIP-Seq analyses were utilized to identify the differentially regulated pathways controlled by PGRA at the time of implantation.

2019-08-29 | GSE86303 | GEO

Uterine glands impact uterine receptivity, luminal fluid homeostasis and blastocyst implantation

Project description:Uterine glands are essential for pregnancy in mice and likely humans, because they secrete or transport bioactive substances that regulate uterine receptivity for blastocyst implantation. In mice, the uterus becomes receptive to blastocyst implantation on day 4, but is refractory by day 5. Here, blastocysts could be recovered from progesterone-induced uterine gland (PUGKO) but not wildtype (WT) mice on day 5 post-mating. Anti-adhesive Muc1 protein and microvilli were present on the luminal epithelium of PUGKO but not WT uteri. A number of known uterine receptivity genes and gland-specific genes were altered in the PUGKO uterus. Next, the uterus and uterine luminal fluid (ULF) were obtained from WT and PUGKO mice on day 3, 4 and 5. Transcriptome analysis revealed that 580 genes were decreased in the PUGKO uterus, however ULF secrotome analysis revealed that many proteins and several amino acids were increased in the PUGKO ULF. Of note, many proteins encoded by many gland-specific genes were not identified in the ULF of WT mice. These results support the ideas that uterine glands secrete factors that regulate ULF homeostasis and interact with other cell types in the uterus to influence uterine receptivity and blastocyst implantation for the establishment of pregnancy.

2016-11-22 | GSE90101 | GEO

Decreased epithelial progesterone receptor A at the window of receptivity is required for preparation of the endometrium for embryo attachment.

Project description:The precise timing of progesterone signaling through its cognate receptor, the progesterone receptor (PGR), is critical for the establishment and maintenance of pregnancy. Loss of PGR expression in the murine uterine epithelium during the preimplantation period is a marker for uterine receptivity and embryo attachment. We hypothesized that the decrease in progesterone receptor A (PGRA) expression is necessary for successful embryo implantation. To test this hypothesis, a mouse model constitutively expressing PGRA (mPgrALsL/+) was generated. Expression of PGRA in all uterine compartments (Pgrcre) or uterine epithelium (Wnt7acre) resulted in infertility with defects in embryo attachment and stromal decidualization. Expression of critical PGRA target genes, indian hedgehog, and amphiregulin (Areg), was maintained through the window of receptivity while the estrogen receptor target gene, the leukemia inhibitory factor (Lif), a key regulator of embryo receptivity, was decreased. Transcriptomic and cistromic analyses of the mouse uterus at day 4.5 of pregnancy identified an altered group of genes regulating molecular transport in the control of fluid and ion levels within the uterine interstitial space. Additionally, LIF and its cognate receptor, the leukemia inhibitory factor receptor (LIFR), exhibited PGR-binding events in regions upstream of the transcriptional start sites, suggesting PGRA is inhibiting transcription at these loci. Therefore, downregulation of the PGRA isoform at the window of receptivity is necessary for the attenuation of hedgehog signaling, transcriptional activation of LIF signaling, and modulation of solutes and fluid, producing a receptive environment for the attaching embryo.

| S-EPMC6225975 | biostudies-literature

MiroRNA expression from pig endometrium exposed to developing pre-implantation embryo versus unfertilized oocytes in vivo

Project description:In vivo evidence suggests a dynamic change of microRNA expression with the phase of the endometrial cycle to achieve endometrial receptivity. In vivo animal studies outlined post – implantation changes in microRNA profiles. Little information is available on the role of microRNAs in the early stages of the embryonic – endometrial dialogue prior to established implantation .Our aim is to investigate the possible role of microRNAs the early endometrial response to the developing pre- attachment blastocyst prior to established implantation

2016-10-04 | GSE87553 | GEO

Transcription profiling of porcine endometrium from chronological stages of psuedopregnancy and estrogen disrupted psuedopregnancy

Project description:Placentation of the conceptus to the surface epithelium is governed through a tightly regulated temporal and spatial window. Premature exogenous steriod exposure causes a shift in the maternal tissue's receptivity and prevents proper placentation. We used Affymetrix microarrays to detail the aberrant changes in uterine gene expression during this window, while removing all conceptus contribution to this dysfunction. Experiment Overall Design: Porcine endometrium was selected at chronological stages of psuedopregnancy and psuedopregnancy post endocrine disrupted early for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain homogeneous populations of normal endometrium at days 13 and 15 of the pseudo pregnant, and endometrium at days 13 and 15 of the endocrine disrupted pseudo pregnant in order to identify the temporal resolution of expression profiles. In brief, days 13 through 15 accounts for the window of attachment during pregnancy in the pig, so we created pseudo pregnant endometrium and obtained samples from days 13 (PIG13UN_TX)and 15 (PIG15UN_TX) of pseudo pregnancy. In the same, we created pseudo pregnant endometrium that was subjected to endocrine disruption of the pseudo pregnancy and collected samples on days 13 (PIG13_TX)and 15 (PIG15_TX) of pseudo pregnancy.

2009-10-10 | E-GEOD-18343 | biostudies-arrayexpress

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