Francisella
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA297804 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| SRR3995548_1.fastq.gz | Fastqsanger.gz | |||
| SRR3995548_2.fastq.gz | Fastqsanger.gz |
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Similar Datasets
Project description:To understand differences of gene expression profiles between Francisella strains RNA profiles of Francisella strains were generated by deep sequencing, in triplicate, using NovaSeq6000. qRT–PCR validation was performed using SYBR Green assays. Our study represents the first detailed differential transcriptomic analysis of Francisella strains , with biologic replicates, generated by RNA-seq technology.
2022-05-31 | GSE174793 | GEO
Project description:The genus Francisella includes several species that cause zoonotic diseases and that have evolved to develop different strategies for survival within the host cells. Despite the critical importance of the diseases caused by members of the genus Francisella, the DNA repair mechanisms of this pathogen remain understudied. In this work, the transcriptional response of Francisella hispaniensis to the DNA-damaging agent ciprofloxacin was studied using RNA sequencing. Among the differentially expressed genes, we identified a S24 family peptidase-encoding gene. Electrophoretic mobility shift assays demonstrated that the product of this gene specifically binds to the palindromic sequence GTG-N11-CAC present in its promoter region, resulting in autoregulation. In silico analyses revealed that this S24 peptidase, here termed FddR, is most likely a co-opted phage repressor that implements a DNA-damage response across the Francisella genus. Our results provide insights into the SOS repair system of Francisella spp. and suggest that the identified S24 family peptidase is a direct regulator of the DNA damage response in F. hispaniensis.
2026-05-15 | GSE308416 | GEO
Project description:The goal of this study is to determine the host response of human epithelial cells during infection with Francisella Tularensis. For this purpose, A549 human epithelial cell line was infected with Francisella tularensis spp. holarctica strain LVS for different times of infection, in duplicates. At different times post infection (0.5/1/3/6/12/24 hours post infection) cells were harvested and total RNA was extracted. RNA-seq libraries were constructed and sequencing of 100bp paired-end was performed on the Illumina NovaSeq 6000 system. Sequencing yielded about 22M reads per sample that were mapped to the human genome (Human: GRCh38) resulting with the identification of 21,066 transcripts. The expression of the infected samples was compared to mock sample, and RNA ratios were clustered using partitioning clustering. This approach allowed clustering of the cellular transcripts into 5 distinct classes based on similarities in temporal expression profiles. We next carried out GO term enrichment analysis for each of these five cluster. Our study represents the first detailed analysis of human epithelial response to Francisella tularensis infection, and provide a framework for comparative investigations of genes and mechanisms that may contribute to the infection.
2020-08-11 | GSE155970 | GEO
Project description:Francisella sp. Genome sequencing and assembly
| PRJNA1033549 | ENA
Project description:Francisella tularensis Genome sequencing and assembly
| PRJNA470596 | ENA
Project description:Francisella possesses a non-canonical T6SS that is essential for efficient phagosomal escape and access to the cytosol of infected macrophages. Using a global and site-specific phosphoproteomic analysis of Francisella we identified here a unique phosphorylation site on IglB, the TssC homologue and a key component of the T6SS contractile sheath. Phosphorylation of the sheath may constitute a previously unrecognized mechanism contributing to the dynamics of assembly-disassembly of the T6SS.
2023-12-20 | PXD012507 | Pride
Project description:Francisella possesses a non-canonical T6SS that is essential for efficient phagosomal escape and access to the cytosol of infected macrophages. Using a global and site-specific phosphoproteomic analysis of Francisella we identified here a unique phosphorylation site on IglB, the TssC homologue and a key component of the T6SS contractile sheath. Phosphorylation of the sheath may constitute a previously unrecognized mechanism contributing to the dynamics of assembly-disassembly of the T6SS.
2019-10-10 | PXD013619 | Pride
Project description:Francisella are pathogenic bacteria whose virulence is linked to their ability to replicate within the host cell cytosol. Entry into the macrophage cytosol activates a host protective multimolecular complex called the inflammasome to release the proinflammatory cytokines IL-1 and IL-18 and trigger caspase-1 dependent cell death. Here we show that cytosolic Francisella induce a type I interferon (IFN) response that is essential for caspase-1 activation, inflammasome mediated cell death, and release of IL-1 and IL-18. Extensive type I IFN dependent cell death resulting in macrophage depletion occurs in vivo during Francisella infection. Type I IFN is also necessary for inflammasome activation in response to cytosolic Listeria but not vacuole localized Salmonella or extracellular ATP. These results show the specific connection between type I IFN signaling and inflammasome activation, two sequential events triggered by recognition of cytosolic bacteria. To our knowledge, this is the first example of positive regulation of inflammasome activation. This connection underscores the importance of cytosolic recognition of pathogens and highlights how multiple innate immunity pathways interact before commitment to critical host responses. Keywords: murine macrophage response to Francisella tularensis subspecies novicida infection
2007-05-23 | GSE7684 | GEO