Project description:Penicillium citrinum X9-4, which was isolated from infected grapes by our laboratory, produced the highest amount of OTA at pH 5 in culture media, and toxin-production was restrained under acidic environment (pH 3). It revealed the possible mechanism of OTA biosynthesis and metabolic regulation in P. citrinum by transcriptomics, and investigated the reason of OTA biosynthesis was restrained in P. citrinum when cultured under acidic environment.
Project description:The saprotrophic fungus P. citrinum is a dorminant leaf-degrading fungus in tobacco industry. The genome-wide exprssion analysis involved in fungal growth was analyzed by using high throughput sequencing (RNA-Seq) on two substrates and time points. Our transcriptional profiles revealed that numerous differentially expressed genes (DEGs), of which involved in metabolism, cell transport and cell rescue, were significantly involved in tobacco-degradation process of P. citrinum.
Project description:In this study, a relative quantitative comparison of proteins that are differentially expressed at two time points during limonene biotransformation (0 and 12 h) is performed using iTRAQ combined with LC–MS/MS. It provides a basis for further study of the molecular mechanism of limonene biotransformation using Penicillium digitatum DSM 62840.