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Xenopus laevis

ABSTRACT: On the relationship of protein and mRNA dynamics in vertebrate embryonic development [RiboZero]

OTHER RELATED OMICS DATASETS IN: PXD002349

PROVIDER: PRJNA298393 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2614100_1.fastq.gz	Fastqsanger.gz
	SRR2614100_2.fastq.gz	Fastqsanger.gz
	SRR2614110_1.fastq.gz	Fastqsanger.gz
	SRR2614110_2.fastq.gz	Fastqsanger.gz
	SRR2614101_1.fastq.gz	Fastqsanger.gz

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Project description:The nephron, functional unit of the vertebrate kidney, is specialized in metabolic wastes excretion and body fluids osmoregulation. Given the high evolutionary conservation of gene expression and segmentation patterning between mammalian and amphibian nephrons, the Xenopus laevis pronephric kidney offers a simplified model for studying nephrogenesis. The Lhx1 transcription factor plays critical roles in kidney development, regulating target gene expression by forming multiprotein complexes with LIM binding protein 1 (Ldb1). However, few Lhx1-Ldb1 cofactors have been identified for this organ formation. By tandem- affinity purification from kidney-induced Xenopus animal caps, we identified single-stranded DNA binding protein 2 (Ssbp2) to interact with the Ldb1-Lhx1 complex. Ssbp2 is expressed in the Xenopus pronephros, and knockdown prevents normal morphogenesis and differentiation of the glomus and the convoluted renal tubules. We demonstrate a role for a member of the Ssbp family in kidney organogenesis and provide evidence of a fundamental function for the Ldb1-Lhx1-Ssbp transcriptional complexes in embryonic development.

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