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Aedes aegypti

ABSTRACT: The midguts transcriptome of Aedes aegypti (Liverpool strain) females blood fed DENV2 NS1 versus those blood fed BSA

PROVIDER: PRJNA298592 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2637683.fastq.gz	Fastqsanger.gz
	SRR2637684.fastq.gz	Fastqsanger.gz
	SRR2637685.fastq.gz	Fastqsanger.gz
	SRR2637686.fastq.gz	Fastqsanger.gz
	SRR2637687.fastq.gz	Fastqsanger.gz

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The midguts transcriptome of Aedes aegypti (Liverpool strain) females blood fed DENV2 NS1 versus those blood fed BSA

Project description:We report the RNA-seq based analyses of the transcriptional changes in the Aedes aegypti midguts transcriptome 4,8,18 hours after blood feeding BSA or DENV2 NS1.

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The midguts transcriptome of Aedes aegypti (Liverpool strain) females knock down AaMesh and feed AaMesh antibody versus those knock down GFP and feed pre-immune.

Project description:We reported the RNA-seq based analyses of the transcriptional changes in the Aedes aegypti midguts knock down 3, 6days, feed antibody 18h transcriptome. Comparison of the midguts transcriptome of Aedes aegypti females at two knockdown time points and one feed condition; GFP dsRNA-3 or -6days: 3 or 6 days after 7day-old mosquitos were microinjected GFP dsRNA AaMesh dsRNA-3 or -6days: 3 or 6 days after 7day-old mosquitos were microinjected AaMesh dsRNA Pre-immune-18h: 18hrs after 7day-old wild type mosquitos were fed with Pre-immune AaMesh antibody-18h: 18hrs after 7day-old wild type mosquitos were fed with AaMesh antibody

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Project description:Rockefeller and Singapore strain Aedes aegypti female mosquitoes differ in the number of bacteria present in the midgut. Females from each strain were either maintained on 3% sucrose solution, fed a sterile blood meal, or fed a blood meal containing a cocktail of bacteria. Differential transcript abundance was compared between females from each strain/treatment combination and a common reference sample pool. The overall goal was to determine how gene expression in Rockefeller females differs from Singapore females in order to better understand why the gut microbiome differs between the strains.

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Transcription profiling of female Anopheles gambiae mosquitoes blood fed on mice infected with P. berghei and treated with chloroquine or untreated

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Project description:Mosquitos were fed sugar water or blood. The blood fed mosquitos were given 6 h or 24 h to digest. The midguts were dissected and prepared for MSP-MS.

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