Project description:Genome-wide DNA methylation of jejunum mucosa was studied using Reduced Representation Bisulfite Sequencing (RRBS) in two laying hen strains, Lohmann Brown-Classic (LB) and Lohmann LSL-Classic (LSL) collected at 10, 16, 24, 30 and 60 weeks of age.
Project description:In this study, we deep-sequenced the transcriptomic profile of the jejunum mucosa in two laying hen strains (Lohmann Brown-Classic and Lohmann LSL-Classic) at 10, 16, 24, 30, and 60 weeks of age. These five time points span the initial, peak and end of the lay periods in which the hens critically experience physiological changes and need optimal diets to meet both growth and egg production requirements. Therefore, a comparative transcriptome profiling of the jejunum mucosa may lead to a better understanding of molecular mechanisms underlying genetics, age and nutrition factors of layers.
Project description:In this study, we deep-sequenced the transcriptomic profile of the ileum mucosa in two laying hen strains (Lohmann Brown-Classic and Lohmann LSL-Classic) at 10, 16, 24, 30, and 60 weeks of age. These five time points span the initial, peak and end of the lay periods in which the hens critically experience physiological changes and need optimal diets to meet both growth and egg production requirements. Therefore, a comparative transcriptome profiling of the ileum mucosa may lead to a better understanding of molecular mechanisms underlying genetics, age and nutrition factors of layers.
Project description:In this study, we deep-sequenced small RNAs of the jejunum mucosa in two laying hen strains (Lohmann Brown-Classic and Lohmann LSL-Classic) at 10, 16, 24, 30, and 60 weeks of age. These five time points span the initial, peak and end of the lay periods in which the hens critically experience physiological changes and need optimal diets to meet both growth and egg production requirements. Therefore, a comparative profiling of miRNAs, as key regulators of gene expression, in the jejunum mucosa may contribute to a better understanding of molecular mechanisms underlying genetics, age and nutrition factors of layers.
Project description:The laying hen is the only spontaneous model of ovarian tumor. But no studies have systematically compared the molecular mechanisms of ovarian cancer in hens and women. We performed RNA sequencing of nine chicken ovarian tumor samples and four normal ovarian samples.