Project description:To understand the effect of salt stress on CbFAD3 transgenic and wild-type tobaccos, we have employed whole genome microarray expression profiling of transgenic and wild-type plants under normal or salt-stress conditons, respectively. We also found the founction of CbFAD3 gene on tobaccos through comparing these data.
2015-10-23 | GSE74260 | GEO
Project description:Transcriptome sequencing of ScDREB5 transgenic Arabidopsis and wild type under normal and salt stress conditions
Project description:Two tobacco transgenic lines over-expressing grapevine polygalacturonase-inhibiting protein (Vvpgip1) vs. WT tobacco under normal growth conditions
Project description:Nicotiana benthamiana is a widely used organism for expression of foreign proteins. To increase protein abundance, overexpression promoters have been developed. However, several proteins cause negative effects on plant growth and development when they are overexpressed. Therefore, overexpression promoters cannot be widely used to generate transgenic plants that produce foreign proteins. In this study, we tried to find stress-inducible promoters which specifically express downstream genes when plants were exposed to stress conditions. By RNA-sequencing analysis of heat- and wound-treated plants, we found that NbHSP20 promoters can be used as a heat-inducible promoters to express foreign proteins in N. benthamiana.
Project description:Comparative analysis of differentially expressed proteins in TaNRX1-2D overexpressing transgenic wheat, TaNRX1-2D-RNAi transgenic wheat, and wild-type wheat before and after salt stress conditions
Project description:we performed endosome proteome enrichment assay (Wang et al., 2023). Endosomes were enriched in the 100,000 g (P100) ultracentrifugation fraction from GFP-StKAKU4 transgenic N. benthamiana leaves during P. infestans infection.
