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Lupinus angustifolius

ABSTRACT: Lupinus angustifolius Genome sequencing and assembly

PROVIDER: PRJNA299755 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR2852994_1.fastq.gz	Fastqsanger.gz
	SRR2852994_2.fastq.gz	Fastqsanger.gz
	SRR2869973_1.fastq.gz	Fastqsanger.gz
	SRR2869973_2.fastq.gz	Fastqsanger.gz

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1 - 5 of 26

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Project description:The aim of this experiment was to compare gene expression in leaves and stems of narrow-leafed lupin (Lupinus angustifolius L.) using Illumina RNA sequencing. Total RNA was isolated from leaves and stems of five narrow-leafed lupin (Lupinus angustifolius L.) genotypes with contrasting alkaloid regulation, including an iucundus low-alkaloid line (83A:476), a bitter Iucundus line (P27255) and three Bryansk low-alkaloid lines carrying the Iucundus-type RAP2-7 allele: 95826 (Bryanskij-35), 95927 (Bryanskij-123) and 95928 (Bryanskij-237/83). Plants, in five replication per genotype, were grown under controlled conditions optimal for growth, and leaf and stem samples were collected at flowering stage.

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PacBio Iso-seq of Lupinus angustifolius L. leaf and stem transcriptomes

Project description:The aim of this experiment was to obtain full length transcript sequences of genes expressed in leaves and stems of narrow-leafed lupin (Lupinus angustifolius L.) using PacBio Iso-Seq. Total RNA was isolated from leaves and stems of five narrow-leafed lupin (Lupinus angustifolius L.) genotypes with contrasting alkaloid regulation, including an iucundus low-alkaloid line (83A:476), a bitter Iucundus line (P27255) and three Bryansk low-alkaloid lines carrying the Iucundus-type RAP2-7 allele: 95826 (Bryanskij-35), 95927 (Bryanskij-123) and 95928 (Bryanskij-237/83). Plants, in five replicates per genotype, were grown under controlled conditions optimal for growth, and leaf and stem samples were collected at flowering stage. For each genotype and organ, total RNA from five biological replicates was pooled in equal amounts and used for PacBio Iso-Seq library preparation and sequencing.

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