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Bos taurus

ABSTRACT: microRNA study of rectal tissue collected from super-shedders vs cattle negative for E. coli O157:H7

PROVIDER: PRJNA301031 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2891580.fastq.gz	Fastqsanger.gz
	SRR2891581.fastq.gz	Fastqsanger.gz
	SRR2891582.fastq.gz	Fastqsanger.gz
	SRR2891583.fastq.gz	Fastqsanger.gz
	SRR2891584.fastq.gz	Fastqsanger.gz

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Bos taurus

Project description:Transcriptome analysis of rectal tissues of E. coli O157 super-shedding and E. coli O157 negative cattle

| PRJNA278944 | ENA

Host mechanisms potentially associated with foodborne pathogen Escherichia coli O157 shedding in feedlot beef cattle

Project description:Cattle shedding > 10e4 CFU of Escherichia coli O157 per g of feces are defined as super-shedders. RNA-sequencing was performed to identify the transcriptome of tissues, including duodenum, proximal jejunum, distal jejunum, distal jejunum, cecum, spiral colon and descending colon of super-shedder cattle in comparison with non-shedders. The total number of genes detected in gut tissues ranged from 16,846 ± 639 (cecum) to 18,137 ± 696 (distal jejunum), and immune functions were enriched for the transcriptomes of small intestinal tissues, reflecting their greater immune activity than large intestine. Totally 351 differentially expression genes were identified between super-shedders and non-shedders, including 101 up-regulated and 250 down-regulated in super-shedders. Differential gene expression analysis suggested increased T-cell responses and cholesterol absorption in distal jejunum and descending colon, and inhibited B-cell maturation in intestines of super-shedders. distal jejunum of super-shedders. Our findings suggested that host genetics and E. coli O157 activities are involved in super-shedding phenomenon.

2017-08-21 | GSE85277 | GEO

Transcriptome analysis of rectal tissues of E. coli O157 super-shedding and E. coli O157 negative cattle

Project description:Total RNA from rectal tissues of E. coli O157 super-shedders and non-shedders were sequenced using Illumina Hiseq 2000 system. For comparative analysis, differentially expressed genes were identified with edgeR. Fifteen of differentially expressed genes were related to innate and adaptive immune functions and were down-regulated in super-shedders, which could be one of reasons for super-shedding phenomenon.

2016-03-20 | GSE67099 | GEO

microRNA study of rectal tissue collected from super-shedders vs cattle negative for E. coli O157:H7

Project description:Total microRNA from rectal tissues of E. coli O157 super-shedders and non-shedders were sequenced using Illumina Hiseq 2000 system. For comparative analysis, differentially expressed microRNAs were identified with edgeR. Two of differentially expressed genes were identified, and one of up-regulated microRNA in super-shedders potentially targets genes related to innate and adaptive immune functions. The findings indicated that microRNA could play a role in super-shedding phenomenon.

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bovine gut metagenome

Project description:Super shedders of Escherichia coli O157:H7 have a unique fecal bacterial community structure

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Bos taurus

Project description:Host mechanisms potentially associated with foodborne pathogen Escherichia coli O157 shedding in feedlot beef cattle

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Transcriptome profiling and Co-expression networks of longissimus dorsi muscle in Gayal（Bos frontalis）and Banna cattle（Bos taurus） with different beef qualities

Project description:The Gayal (Bos frontalis) is a rare semi-domesticated cattle in China. Gayal has typical beef body shape and good meat production performance. Compared with other cattle species, it has the characteristics of tender meat and extremely low fat content. To explore the underlying mechanism responsible for the differences of meat quality between different breeds, the longissimus dorsi muscle (LM) from Gayal and Banna cattle (Bos taurus) were investigated using transcriptome analysis. The gene expression profiling identified 638 differentially expressed genes (DEGs) between LM muscles from Gayal and Banna cattle. Gene Ontology (GO) enrichment of biological functions and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the gene products were mainly involved in the PPAR signaling pathway, lipid metabolism and amino acid metabolism pathway. Protein-protein interaction（PPI） network analysis showed APOB, CYP7A1, THBS2, ITGAV, IGFBP1 and IGF2R may have great impact on meat quality characteristics of Gayal. Moreover, three transcription factors, FOXA2, NEUROG2, and RUNX1, which may affect meat quality by regulating the expression of genes related to muscle growth and development have also been found. In summary, our research reveals the molecular mechanisms that cause Gayal meat quality characteristics. It will contribute to improving meat quality of cattle through molecular breeding.

2024-09-01 | GSE164462 | GEO

Bos taurus

Project description:MicroRNAomes of cattle intestinal tissues revealed possible miRNA regulated mechanisms involved in Escherichia coli O157 fecal shedding

| PRJNA380253 | ENA

Indole-3-Acetaldehyde Inhibits Escherichia coli O157:H7 Biofilm Formation by Reducing Curli Formation

Project description:Pathogenic biofilms have been associated with persistent infections due to their high resistance to antimicrobial agents. To identify non-toxic biofilm inhibitors for enterohemorrhagic Escherichia coli O157:H7, indole-3-acetaldehyde was used and reduced E. coli O157:H7 biofilm formation. Global transcriptome analyses revealed that indole-3-acetaldehyde most repressed two curli operons, csgBAC and csgDEFG, and induced tryptophanase (tnaAB) in E. coli O157:H7 biofilm cells. Electron microscopy showed that indole-3-acetaldehyde reduced curli production in E. coli O157:H7. Together, this study shows that Actinomycetales are an important resource of biofilm inhibitors as well as antibiotics.

2012-07-05 | GSE30424 | GEO

Transcriptional analysis of Escherichia coli O157:H7 delta yedL in MEM-HEPES

Project description:Deletion of yedL was found to signifcantly decrease type three secretion in EHEC O157:H7. Transcriptional profiles of Escherichia coli O157: H7 and the isogenic yedL mutant were generated and compared.

2017-07-24 | GSE15265 | GEO

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