Project description:we report the application of single cell RNAseq analysis of mouse lung from E20.5 Nkx2-1-/- rat (mutant), and mouse-rat chimeric lung from E20.5 wt rat (control).
Project description:Rat ES cells were derived using 3I medium from E4.5 blastocysts. Rat embryonic fibroblast cells were derived form E14.5 embryos. To analyze the mechanism under the selfrenewal of rat ES cells, microarrays were used for the genome wide analysis of gene expressoin profiles in rat ES cells. Rat embryonic fibroblast cells and mouse ES cells were tested at same time as control. Our results from clustering analysis demonstrated that the gene expression profile of rat ES cells resembles mouse ES cells, but not REFs. Keyword: 3I medium; rat embryonic stem cells; mouse ES cells; rat embryonic fibroblast cells
Project description:We performed single cell RNA-seq to quantify gene expression in forebrain tissue from the wild-type mouse, rat, and chimeric mouse. Single cell data from WT mouse or rat was used to perform integrative analysis for identifying rat cells and mouse cells in the chimaeras, respectively.
