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Homo sapiens

ABSTRACT: The role of PRMT5/WDR77 complex in promoting breast cancer oncogenesis [RNA-Seq]

PROVIDER: PRJNA305261 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2977060_1.fastq.gz	Fastqsanger.gz
	SRR2977060_2.fastq.gz	Fastqsanger.gz
	SRR2977061_1.fastq.gz	Fastqsanger.gz
	SRR2977061_2.fastq.gz	Fastqsanger.gz
	SRR2977058_1.fastq.gz	Fastqsanger.gz

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The role of PRMT5/WDR77 complex in promoting breast cancer oncogenesis [RNA-Seq]

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Project description:The PRMT5/WDR77 complex is known to regulate alternative splicing through the symmetric dimethylation of spliceosome proteins in the cytoplasm. Overexpression of this complex is observed in different types of cancer including breast cancer, where it shows increased nuclear accumulation.To understand the nuclear role and the chromatin binding properties of PRMT5 we performed genome-wide ChIP-Seq analysis in the breast cancer cell line MDA-MB-231.

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Project description:Analysis of gene expression in lung and prostate cancer cells expressing non-target (NT), p44/wdr77. or PRMT5 shRNA. Results provide important information on how p44/wdr77 and PRMT5 control cellular proliferation. Total RNA obtained from cells expressing NT, p44/wdr77, or PRMT5 shRNA.

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Project description:CRISPR was utilized to deplete either PRMT5 or WDR77 in HSC-5 cells. Subsequently, RNA-seq was performed to identify changes in the transcriptome following the loss of PRMT5 or WDR77 in these cells. Critically, Gene Set Enrichment Analysis (GSEA) was conducted to screen and identify a list of genes co-regulated by PRMT5 and WDR77.

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