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The chicken erythrocyte epigenome

ABSTRACT: The chicken erythrocyte epigenome

PROVIDER: PRJNA305808 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2983620.fastq.gz	Fastqsanger.gz
	SRR2983621.fastq.gz	Fastqsanger.gz
	SRR2983612.fastq.gz	Fastqsanger.gz
	SRR2983622.fastq.gz	Fastqsanger.gz
	SRR2983613.fastq.gz	Fastqsanger.gz

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The chicken immature erythrocyte transcriptome

Project description:Transcriptional regulation is impacted by multiple layers of genome organization. Here, we identified and mapped out all the transcriptionally active chromosomal domains in the chicken immature erythrocyte genome, including the known Î²- and Î±-globin domains, by combining a powerful chromatin fractionation method with next generation DNA and RNA sequencing. Two biological replicates of total RNA were sequenced to characterize genes in chicken erythrocyte cells

2016-07-03 | E-GEOD-75848 | biostudies-arrayexpress

The chicken immature erythrocyte transcriptome

2016-01-01 | GSE75848 | GEO

Genome-wide mapping of H3K4me3 and H3K27ac in chicken immature erythrocyte cells

Project description:Transcriptional regulation is impacted by multiple layers of genome organization. Here, we report that highly expressed genes were associated with H3K4me3 and H3K27ac.Our data provide a genome-wide profile of chromatin signatures in relation to expression levels in chicken immature erythrocytes. Examination of two different histone modifications in immature erythrocyte cells

2016-07-03 | E-GEOD-75845 | biostudies-arrayexpress

The chicken erythrocyte epigenome.

Project description:BackgroundTranscriptional regulation is impacted by multiple layers of genome organization. A general feature of transcriptionally active chromatin is sensitivity to DNase I and association with acetylated histones. However, very few of these active DNase I-sensitive domains, such as the chicken erythrocyte β-globin domain, have been identified and characterized. In chicken polychromatic erythrocytes, dynamically acetylated histones associated with DNase I-sensitive, transcriptionally active chromatin prevent histone H1/H5-induced insolubility at physiological ionic strength.ResultsHere, we identified and mapped out all the transcriptionally active chromosomal domains in the chicken polychromatic erythrocyte genome by combining a powerful chromatin fractionation method with next-generation DNA and RNA sequencing. Two classes of transcribed chromatin organizations were identified on the basis of the extent of solubility at physiological ionic strength. Highly transcribed genes were present in multigenic salt-soluble chromatin domains ranging in length from 30 to over 150 kb. We identified over 100 highly expressed genes that were organized in broad dynamically highly acetylated, salt-soluble chromatin domains. Highly expressed genes were associated with H3K4me3 and H3K27ac and produced discernible antisense transcripts. The moderately- and low-expressing genes had highly acetylated, salt-soluble chromatin regions confined to the 5' end of the gene.ConclusionsOur data provide a genome-wide profile of chromatin signatures in relation to expression levels in chicken polychromatic erythrocytes.

| S-EPMC4879735 | biostudies-literature

The chicken erythrocyte epigenome

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-01-01 | GSE75955 | GEO

The chicken erythrocyte epigenome

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-07-03 | E-GEOD-75955 | biostudies-arrayexpress

Genome-wide mapping of immature erythrocyte transcribed chromosomal domains (F1-seq)

2016-01-01 | GSE75849 | GEO

Genome-wide mapping of H3K4me3 and H3K27ac in chicken immature erythrocyte cells

2016-01-01 | GSE75845 | GEO

megamiR, microRNA expression profiles during megakaryocyte-erythrocyte progenitor (MEP) differentiation

Project description:This collection contains microRNA expression profiling data for samples purified from umbilical cord blood, belonging to one of the followiing populations: MEP, MEGA1, MEGA2, ERY1, ERY2, ERY3. MEP: megakaryocyte-erythrocyte precursors (defined by CD34+ CD38+ IL-3Ra- CD45RA- ); MEGA1: megakaryocyte population 1 (defined by CD34+ CD61+ CD41+ CD45- ); MEGA2: megakaryocyte population 2 (defined by CD34- CD61+ CD41+ CD45- ); ERY1: erythrocyte population 1 (defined by CD34+ CD71+ GlyA- ); ERY2: erythrocyte population 2 (defined by CD34- CD71+ GlyA- ); ERY3: erythrocyte population 3 (defined by CD34- CD71+ GlyA+ ). Keywords: microRNA, miRNA, MEP, megakaryocyte, erythrocyte, lineage specification

2008-06-10 | GSE10593 | GEO

Genome-wide mapping of immature erythrocyte transcribed chromosomal domains (F1-seq)

Project description:Transcriptional regulation is impacted by multiple layers of genome organization. Here, we identified and mapped out all the transcriptionally active chromosomal domains in the chicken immature erythrocyte genome, including the known Î²- and Î±-globin domains, by combining a powerful chromatin fractionation method with next generation DNA and RNA sequencing. To characterize transcriptionally active chromatin domains, we used two biological replicates. Each replicate set has one control SE fraction and one transcriptionally active chromatin fraction (F1)

2016-07-03 | E-GEOD-75849 | biostudies-arrayexpress

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