Arabidopsis thaliana
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA306773 | ENA |
REPOSITORIES: ENA
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Project description:Nitrogen and light are two major regulators of plant metabolism and development. While genes involved in the control of each of these signals have begun to be identified, regulators that integrate gene responses to nitrogen and light signals have yet to be determined. Here, we evaluate the role of bZIP1, a transcription factor involved in light and nitrogen sensing, by exposing wild-type (WT) and bZIP1 T-DNA null mutant plants to a combinatorial space of N and L treatment conditions. We use ANOVA analysis combined with clustering and Boolean modeling, to evaluate the role of bZIP1 in mediating L and N signaling genome-wide.
2010-08-25 | GSE21601 | GEO
Project description:Testing Arabidopsis for the presence of arbuscular mycorrhizal signalling pathways
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Project description:Nitrogen and light are two major regulators of plant metabolism and development. While genes involved in the control of each of these signals have begun to be identified, regulators that integrate gene responses to nitrogen and light signals have yet to be determined. Here, we evaluate the role of bZIP1, a transcription factor involved in light and nitrogen sensing, by exposing wild-type (WT) and bZIP1 T-DNA null mutant plants to a combinatorial space of N and L treatment conditions. We use ANOVA analysis combined with clustering and Boolean modeling, to evaluate the role of bZIP1 in mediating L and N signaling genome-wide. Arabidopsis thaliana were growth on basal MS salts (custom-made; GIBCO) with 0.5 mM KNO3, 3 mM sucrose and 0.8% BactoAgar at pH 5.7. After 14 days under long-day (16 h light: 8 h dark) conditions with light intensity of 50 μE.m-2.s-1 and at 22°C, plants were transferred to new plates containing 20 mM KNO3 and 20 mM NH4NO3 (referred here as 1xN: concentrations in MS media) in the absence or presence of light for 2 h at the start of their light cycle.
2010-08-25 | E-GEOD-21601 | biostudies-arrayexpress
Project description:AtbZIP16 integrates light and hormone signaling pathways to regulate Arabidopsis early seedling development
| PRJNA146303 | ENA
Project description:Systemic signalling of irradiance and CO2 concentration in Arabidopsis (Treatment 3: Ambient CO2 and Low Light)
| PRJNA96833 | ENA
Project description:Systemic signalling of irradiance and CO2 concentration in Arabidopsis (Treatment 1: Ambient CO2 and Ambient Light)
| PRJNA96829 | ENA
Project description:Systemic signalling of irradiance and CO2 concentration in Arabidopsis (Treatment 2: Elevated CO2 and Ambient Light)
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Project description:Transcriptome analysis suggests that lrb123 and pif3 mutants have similar gene expression profiles with pif4 mutants, suggesting that LRBs and PIF3 have overlapping functions with PIF4. Further analysis revealed that most of the genes induced by PIF4 in a temperature-dependent manner are strongly downregulated. These genes belong to the Auxin/BR growth hormone pathways, wherein genes involved in auxin/BR biosynthesis and signalling are severely perturbed in response to 27°C compared to 22°C. These results indicate that LRBs and PIF3-mediated induction of PIF4 target genes in response to warm temperature is likely through PIF4. Moreover, our data also demonstrate that both LRBs and PIF3 inhibit the expression of light-responsive genes in response to warmth, further highlighting the role of LRBs and PIF3 in promoting thermomorphogenesis, is by directly promoting growth-responsive genes, and second by inhibiting light signalling genes. Together, we conclude that LRBs and PIF3 are novel components of the thermosensory pathway and are essential to promote themomorhogensis, likely by enhancing the PIF4 activity and down gene expression.
2025-06-03 | GSE281310 | GEO
Project description:Arabidopsis thaliana ecotypes Columbia (Col-0) (wild type: WT) was used in this study. After sterilization, the seeds were placed on Murashige and Skoog medium supplemented with 2% (w/v) sucrose for 10 days and then the seedling were transferred to soil under 16 hours light (22°C) / 8 hours dark (18°C) period in growth chamber at a light intensity of 120?150 µmol m-2 s-1. 20-day-old Arabidopsis leaves without bolting were immediately frozen in liquid nitrogen for RNA and protein and metabolites extraction. Leaves were harvested at three different time points: t = 0 hr (end of night), t = 1 hr (one hour after light turn on) and t = 8 hr (eight hours after light turn on), respectively.
2016-01-20 | GSE57791 | GEO