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Analysis of Hybrid Incompatability between C nigoni and C briggsae by mRNA sequencing

ABSTRACT: Analysis of Hybrid Incompatability between C nigoni and C briggsae by mRNA sequencing

OTHER RELATED OMICS DATASETS IN: GSE76306

PROVIDER: PRJNA306868 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3052000_1.fastq.gz	Fastqsanger.gz
	SRR3052000_2.fastq.gz	Fastqsanger.gz
	SRR3052001_1.fastq.gz	Fastqsanger.gz
	SRR3052001_2.fastq.gz	Fastqsanger.gz
	SRR3052002_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

Analysis of Hybrid Incompatability between C nigoni and C briggsae by mRNA sequencing

Project description:Two introgression strains (ZZY10307 and ZZY10330) of C. briggsae onto the X chromosome of C. nigoni results in male sterility. In order to determine the cause, we sequenced the mRNAs from young adult males from these two strains, and compared to fertile males of the two parent species (AF16 and JU1421). Two wild-type female samples were also included as platform QC.

2016-05-11 | GSE76306 | GEO

Analysis of Hybrid Incompatability between C nigoni and C briggsae

Project description:Analysis of Hybrid Incompatability between C nigoni and C briggsae

| PRJNA305287 | ENA

Analysis of Hybrid Incompatability between C nigoni and C briggsae

Project description:An introgression of C. briggsae onto the X chromosome of C briggsae results in male sterility. In order to determine the cause, we sequenced small RNAs from this strain and compared to fertile males of the two parent species.

2016-05-11 | GSE75763 | GEO

A Genome-wide hybrid incompatibility landscape between Caenorhabditis briggsae and C. nigoni.

Project description:Systematic characterization of ẖybrid incompatibility (HI) between related species remains the key to understanding speciation. The genetic basis of HI has been intensively studied in Drosophila species, but remains largely unknown in other species, including nematodes, which is mainly due to the lack of a sister species with which C. elegans can mate and produce viable progeny. The recent discovery of a C. briggsae sister species, C. nigoni, has opened up the possibility of dissecting the genetic basis of HI in nematode species. However, the paucity of dominant and visible marker prevents the efficient mapping of HI loci between the two species. To elucidate the genetic basis of speciation in nematode species, we first generated 96 chromosomally integrated GFP markers in the C. briggsae genome and mapped them into the defined locations by PCR and Next-Generation Sequencing (NGS). Aided by the marker, we backcrossed the GFP-associated C. briggsae genomic fragments into C. nigoni for at least 15 generations and produced 111 independent introgressions. The introgression fragments cover most of the C. briggsae genome. We finally dissected the patterns of HI by scoring the embryonic lethality, larval arrest, sex ratio and male sterility for each introgression line, through which we identified pervasive HI loci and produced a genome-wide landscape of HI between the two nematode species, the first of its type for any non-Drosophila species. The HI data not only provided insights into the genetic basis of speciation, but also established a framework for the possible cloning of HI loci between the two nematode species. Furthermore, the data on hybrids confirmed Haldane's rule and suggested the presence of a large X effect in terms of fertility between the two species. Importantly, this work opens a new avenue for studying speciation genetics between nematode species and allows parallel comparison of the HI with that in Drosophila and other species.

| S-EPMC4334894 | biostudies-literature

Genomic basis of recombination suppression in the hybrid between Caenorhabditis briggsae and C. nigoni.

Project description:DNA recombination is required for effective segregation and diversification of genomes and for the successful completion of meiosis. Recent studies in various species hybrids have demonstrated a genetic link between DNA recombination and speciation. Consistent with this, we observed a striking suppression of recombination in the hybrids between two nematodes, the hermaphroditic Caenorhabditis briggsae and the gonochoristic C. nigoni. To unravel the molecular basis underlying the recombination suppression in their hybrids, we generated a C. nigoni genome with chromosome-level contiguity and produced an improved C. briggsae genome with resolved gaps up to 2.8 Mb. The genome alignment reveals not only high sequence divergences but also pervasive intra- and inter-chromosomal sequence re-arrangements between the two species, which are plausible culprits for the observed suppression. Comparison of recombination boundary sequences suggests that recombination in the hybrid requires extensive sequence homology, which is rarely seen between the two genomes. The new genomes and genomic libraries form invaluable resources for studying genome evolution, hybrid incompatibilities and sex evolution for this pair of model species.

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Caenorhabditis

Project description:Analysis of Hybrid Incompatability between C nigoni and C briggsae by mRNA sequencing

| PRJNA306406 | ENA

Caenorhabditis briggsae strain:AF16

Project description:Chromosomal-level genome assembly and annotation for C. briggsae and C. nigoni

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Caenorhabditis nigoni

Project description:Caenorhabditis nigoni overview

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C. briggsae developmental timecourse

Project description:To identify differences in gene expression patterns between C. elegans and C. briggsae we designed whole-genome species-specific microarrays.

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