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Mus musculus

ABSTRACT: Histone H3 Lysine 27 methylation Regulates Skeletal Growth by Suppressing Wnt and TGF-β Signaling

PROVIDER: PRJNA307511 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3080860_1.fastq.gz	Fastqsanger.gz
	SRR3080860_2.fastq.gz	Fastqsanger.gz
	SRR3080861_1.fastq.gz	Fastqsanger.gz
	SRR3080861_2.fastq.gz	Fastqsanger.gz

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Histone H3 lysine 27 trimethylation of miRNA genes in HMEC and HMF

Project description:Normal cell type specific histone H3 lysine 27 trimethylation of miRNA genes. HMEC and HMF represent two distinct differentiated cell type present in mammary gland each with a distinct phenotype, a distinct epigenotype as well as distinct miRNA expression pattern. The aim of the study was to determine how epigenetic modifications including histone H3 lysine 27 trimethylation affect miRNA expression. Two cell types HMEC vs. HMF. Biological replicates: 3 pairs of HMEC-HMF of 3 distinct genotypes. Immunoprecipitation using anti-histone H3 trimethylated at lysine 27 (07-449, Millipore).

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ACSS2-coupled KAT2A regulates histone H3 lactylation in gene promoter regions and gene expression

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Enhancer Mapping in Chronic Lymphocytic Leukemia

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Project description:Transforming growth factor-β (TGF-β) signaling stimulates cell movement and plasticity by inducing epithelial-to-mesenchymal transition (EMT). This process is aberrantly activated in cancer and facilitates tumor cell migration, invasion, metastasis, and therapy resistance. In this study, we identified the lncRNA eSNAI1 (SCREEM2) as a potent activator of TGF-β/SMAD signaling and SNAI1 expression. eSNAI1 depletion reduces TGF-β-induced EMT, migration, in vivo extravasation, stemness, and chemotherapy resistance of breast cancer cells. eSNAI1 promotes TGF-β/SMAD signaling by inhibiting TGF-β type I receptor polyubiquitination and proteasomal degradation. eSNAI1 stimulates the expression of the nearby gene SNAI1, which encodes the EMT transcription factor SNAI1 that facilitates TGF-β/SMAD signaling by retaining the inhibitory SMAD7 in the nucleus. Mechanistically, eSNAI1 acts as an enhancer RNA (eRNA) to potentiate SNAI1 expression through in-cis regulation. Furthermore, we uncovered that eSNAI1 interacts with and reinforces the binding of the co-activator bromodomain-containing protein 4 (BRD4) to the H3 lysine 27 acetylation (H3K27ac)-enriched SNAI1 enhancer region. Our findings identify eSNAI1 as a potent activator of TGF-β signaling and a promising therapeutic target to mitigate overactive TGF-β signaling and EMT in cancer cells.

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Neurospora

Project description:Regional control of histone H3 lysine 27 methylation in Neurospora

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Histone H3 lysine 27 trimethylation of miRNA genes in HMEC and HMF

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