Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA308317 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR3095300.fastq.gz | Fastqsanger.gz | |||
| SRR3095310.fastq.gz | Fastqsanger.gz | |||
| SRR3095320.fastq.gz | Fastqsanger.gz | |||
| SRR3095330.fastq.gz | Fastqsanger.gz | |||
| SRR3095340.fastq.gz | Fastqsanger.gz |
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Project description:The G-quadruplex is an alternative DNA structural motif that is considered to be functionally important in the mammalian genome. Herein, we address the hypothesis that G-quadruplex structures can exist within double-stranded genomic DNA using a G-quadruplex-specific probe. An engineered antibody is employed to enrich for DNA containing G-quadruplex structures, followed by deep sequencing to detect and map G-quadruplexes at high resolution in genomic DNA from human breast adenocarcinoma cells. Our high sensitivity structure-based pull-down strategy enables the isolation of genomic DNA fragments bearing a single as well as multiple G-quadruplex structures. Stable G-quadruplex structures are found in sub-telomeres, gene bodies and gene regulatory regions. For a sample of identified target genes, we show that G-quadruplex stabilizing ligands can modulate transcription. These results confirm the existence of G-quadruplex structures and their persistence in human genomic DNA.
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