Project description:Transcriptome profiling of human anaplastic meningioma IOMM-Lee cells following silencing of HuR (Human antigen R) gene expression by siRNA
Project description:Differential gene expression profiles between SUM149 cells transfected with control siRNA and SUM149 cells transfected with siRNA targeting tarzarotene-induced gene 1 (TIG1)
Project description:Changes in the cellular transcriptome, proteome and phosphorylated proteome after knockdown of ERK5 protein in HCT116 cells using PROTACs and siRNA, respectively.
Project description:Gene expression profiling comparisons of Neuro2a transfected with either mKIAA1583 (Tmem132a) siRNA or control (non-specific) siRNA. Keywords: siRNA, gene expression
Project description:A GAPDH siRNA was overexpressed in HeLa cells and microarray expression profiling was performed to experimentally identify the off-targets of this siRNA at the transcriptome level. Among all predicted siRNA off-targets by MirTarget2, the majority were downregulated by at least 10% (i.e. less than 90% remaining gene expression). Moreover, more than one fifth were downregulated by at least 30%. The gene knockdown data is consistent with previous observations that siRNA off-targets are generally silenced at a more moderate level as compared to silencing of the single intended target