Project description:A microarray analysis (transcriptome analysis) was conducted to examine estrogen-mediated gene expression in the arcuate nucleus (ARC) and paraventricular nucleus (PVN) of the hypothalamus, as well as the nucleus of the solitary tract (NTS) in female rats (Rattus norvegicus). All procedures were approved by the Oklahoma State University - Center for Health Sciences IACUC (protocol #2021-1278). Rats underwent ovariectomy, recovered, and were then treated with either 17β-estradiol (EB) or an oil vehicle (Oil). Following euthanasia, mRNA was extracted and submitted to Thermo Fisher Scientific for microarray analysis using the Clariom S Rat Pico Assay.
