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Mus musculus

ABSTRACT: OSKM induce extraembryonic endoderm stem (iXEN) cells in parallel to iPS cells

OTHER RELATED OMICS DATASETS IN: E-GEOD-77550

PROVIDER: PRJNA310771 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3143520.fastq.gz	Fastqsanger.gz
	SRR3143530.fastq.gz	Fastqsanger.gz
	SRR3143540.fastq.gz	Fastqsanger.gz
	SRR3143521.fastq.gz	Fastqsanger.gz
	SRR3143531.fastq.gz	Fastqsanger.gz

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Similar Datasets

E-GEOD-77550

Project description:OSKM induce extraembryonic endoderm stem (iXEN) cells in parallel to iPS cells

2019-05-14 | E-GEOD-77550 | ExpressionAtlas

OSKM induce extraembryonic endoderm stem (iXEN) cells in parallel to iPS cells

Project description:While the reprogramming factors OCT4, SOX2, KLF4, and MYC (OSKM) can reactivate the pluripotency network in terminally differentiated cells, they also regulate expression of non-pluripotency genes in other contexts, such as the mouse primitive endoderm. The primitive endoderm is an extraembryonic lineage established alongside the pluripotent epiblast in the blastocyst, and is the progenitor pool for extraembryonic endoderm stem (XEN) cells. Several studies have shown that endodermal genes are upregulated in fibroblasts undergoing reprogramming, although whether endodermal genes promote or inhibit acquisition of pluripotency is unclear. We show that, in fibroblasts undergoing conventional reprogramming, OSKM-induced expression of endodermal genes leads to formation of induced XEN (iXEN) cells, which possess key properties of blastocyst-derived XEN cells, including morphology, transcription profile, self-renewal, and multipotency. Our data show that iXEN cells arise in parallel to iPS cells, indicating that OSKM are sufficient to drive cells to two distinct fates during reprogramming.

2016-03-03 | GSE77550 | GEO

OSKM induce extraembryonic endoderm stem (iXEN) cells in parallel to iPS cells

2016-03-03 | E-GEOD-77550 | biostudies-arrayexpress

OCT4 is expressed in extraembryonic endoderm stem (XEN) cell progenitors during somatic cell reprogramming

Project description:During development, progenitors of embryonic stem (ES) and extraembryonic endoderm stem (XEN) cells are concomitantly specified within the inner cell mass (ICM) of the mouse blastocyst. Similarly, XEN cells are induced (iXEN cells) alongside induced pluripotent stem (iPS) cells following overexpression ofOct4,Sox2,Klf4andMyc(OSKM) during somatic cell reprogramming. It is unclear how or why this cocktail produces both stem cell types, but OCT4 has been associated with non-pluripotent outcomes. In this report, we show that, during OSKM reprogramming, many individualOct4-GFP-expressing cells are fated to become iXEN cells. Interestingly, SKM alone was also sufficient to induce iXEN cell formation, likely via activation of endogenousOct4.These observations indicate that iXEN cell formation is not strictly an artifact ofOct4overexpression. Moreover, our results suggest that a pathway to XEN may be an integral feature of establishing pluripotency during reprogramming, as in early embryo development.

2024-02-15 | GSE244818 | GEO

Mus musculus

Project description:Expression and ChIP-seq analyses of embryonic stem cells, extraembryonic endoderm stem cells, and trophoblast stem cells

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Project description:Genome-Wide Sox17 Binding Sites in Mouse Extraembryonic Endoderm and Embryonic Stem Cells

| PRJNA120621 | ENA

Mus musculus

Project description:BMP4 signalling directs primitive endoderm-derived XEN cells to an extraembryonic visceral endoderm identity

| PRJNA147561 | ENA

Mus musculus

Project description:BMP and Activin treatment of mouse extraembryonic endoderm (XEN) cells

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Project description:Comparative Analysis of Extraembryonic Endoderm Cells with Cardiac Inducing Ability

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Mus musculus

Project description:Gata6 potently initiates reprogramming of pluripotent and differentiated cells to extraembryonic endoderm stem cells [time-course microarray]

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