Brachyspira hyodysenteriae WA1
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA31263 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Molecular characterisation of antimicrobial resistance in Brachyspira hyodysenteriae, aetiological agent for swine dysentery
| PRJEB24023 | ENA
Project description:Using RNA-seq, we investigated for the first time the in vivo transcriptome of colonic mucosa in 16 control pigs and 16 pigs experimentally infected with Brachyspira hyodysenteriae, which were euthanized at different stages of the disease: 8 during a preclinical stage characterized by bacterial shedding without clinical signs (early infection group), and 8 during an acute stage presenting with dysentery (acute infection group). No significant differences in gene expression were observed between the control and early infection groups, except for an overexpression of matrix metalloproteinases (MMPs), which may be associated with early ulceration of the colonic epithelium. The acute infection group showed a significant upregulation of the S100A8, S100A9 and S100A12 proteins, but most of the host biological processes were downregulated, particularly those related to the immune response, such as chemokines and cytokines signalling or immune cells chemotaxis and migration. Accordingly, the prediction of cell types associated with the detected transcripts revealed a depletion of immune cells, except neutrophils, and an enrichment of stromal cells in the acute infection group. Marked alterations in mucin gene expression were observed in this group, including overexpression of MUC5AC, MUC20, and GCNT3, a trend toward increased MUC2, and downregulation of MUC1 and MUC3. Although no significant changes were detected in the early infection group, a similar trend was observed. These findings suggest that B. hyodysenteriae may suppress host immune responses, particularly through inhibition of the IFNγ signalling pathway, accompanied by a depletion of cellular responses, except for neutrophils, and promotion of tissue damage via overexpression of multiple MMPs, even at early stages of the disease. Significant alterations in mucin composition were observed during the acute stage, likely driven by the overexpression of S100 proteins and activation of the SPDEF pathway. This study proposes several mechanisms associated with the pathophysiology of swine dysentery, revealing key aspects of host–pathogen interactions, particularly the host response to B. hyodysenteriae infection.
2025-07-28 | GSE302242 | GEO
Project description:Brachyspira hyodysenteriae overview
| PRJNA37005 | ENA
Project description:Brachyspira hyodysenteriae Genome sequencing and assembly
| PRJNA272555 | ENA
Project description:Brachyspira hyodysenteriae Genome sequencing and assembly
| PRJNA324617 | ENA
Project description:Brachyspira hyodysenteriae Genome sequencing and assembly
| PRJNA326554 | ENA
Project description:The causative agent of swine mycoplasmosis.
| PRJNA13120 | ENA
Project description:Brachyspira hyodysenteriae and Brachyspira pilosicoli are well-known intestinal pathogens in pigs. B. hyodysenteriae is the causative agent of swine dysentery, a disease with an important impact on pig production while B. pilosicoli is responsible of a milder diarrheal disease in these animals, porcine intestinal spirochetosis. Recent sequencing projects have provided information for the genome of these species facilitating the search of vaccine candidates using reverse vaccinology approaches. However, practically no experimental evidence exists of the actual gene products being expressed and of those proteins exposed on the cell surface or released to the cell media. Using a cell-shaving strategy and a shotgun proteomic approach we carried out a large-scale characterization of the exposed proteins on the bacterial surface in these species as well as of peptides and proteins in the extracellular medium. The study included three strains of B. hyodysenteriae and two strains of B. pilosicoli and involved 148 LC-MS/MS runs on a high resolution Orbitrap instrument. Overall, we provided evidence for more than 29,000 different peptides pointing to 1625 and 1338 different proteins in B. hyodysenteriae and B. pilosicoli, respectively. Many of the most abundant proteins detected corresponded to described virulence factors and vaccine candidates. The level of expression of these proteins, however, was different among species and strains, stressing the value of determining actual gene product levels as a complement of genomic-based approaches for vaccine design.
| S-EPMC4955376 | biostudies-literature
Project description:Causative agent of Glasser's disease in swine
| PRJNA20127 | ENA
Project description:Causative agent of enzootic pneumonia in swine.
| PRJNA10639 | ENA