Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Pioneering activity of the C-terminal domain of EBF1 shapes the chromatin landscape for B cell programming

ABSTRACT: Pioneering activity of the C-terminal domain of EBF1 shapes the chromatin landscape for B cell programming

PROVIDER: PRJNA314123 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR3222600_1.fastq.gz	Fastqsanger.gz
	SRR3222600_2.fastq.gz	Fastqsanger.gz
	SRR3222601_1.fastq.gz	Fastqsanger.gz
	SRR3222601_2.fastq.gz	Fastqsanger.gz
	SRR3222602_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 40

Similar Datasets

Dynamic EBF1 occupancy directs sequential epigenetic and transcriptional events in B cell programming [EBF1-ChIP-Seq-Cre]

Project description:EBF1 is essential for B cell specification and commitment. To explore the dynamics of EBF1 initiated B cell programming, we performed EBF1 ChIP-seq, ATAC-seq, bisulfite-seq, RNA-seq and several histone ChIP-seq analyses at different stages of the transition from Ebf1-/- pre-pro-B to pro-B triggered by EBF1 restoration. We also performed Pax5 ChIP-seq in Ebf1-/- pre-pro-B cell and EBF1-restored pro-B cell to study the pioneering function of EBF1 that allows other transcription factors to access certain chromatin sites.

2018-02-20 | GSE107236 | GEO

Dynamic EBF1 occupancy directs sequential epigenetic and transcriptional events in B cell programming [EBF1-ChIP-Seq-Tet-On]

2018-02-20 | GSE107237 | GEO

Dynamic EBF1 occupancy directs sequential epigenetic and transcriptional events in B cell programming [Bisulfite-Seq-Cre]

2018-02-20 | GSE107241 | GEO

Dynamic EBF1 occupancy directs sequential epigenetic and transcriptional events in B cell programming [RNA-Seq-Cre]

2018-02-20 | GSE107240 | GEO

Dynamic EBF1 occupancy directs sequential epigenetic and transcriptional events in B cell programming [ATAC-Seq-Cre]

2018-02-20 | GSE107234 | GEO

Dynamic EBF1 occupancy directs sequential epigenetic and transcriptional events in B cell programming [Histone-ChIP-Seq-Cre]

2018-02-20 | GSE107238 | GEO

Dynamic EBF1 occupancy directs sequential epigenetic and transcriptional events in B cell programming [ATAC-Seq-Tet-On]

2018-02-20 | GSE107235 | GEO

Dynamic EBF1 occupancy directs sequential epigenetic and transcriptional events in B cell programming [Pax5-ChIP-Seq-Tet-On]

2018-02-20 | GSE107239 | GEO

ChIP-seq of pro-B cells to test the role of phase separation in EBF1 pioneering function

Project description:EBF1 is a transcription factor crucial for early B cell development. The phase separation ability of the EBF1 C-terminal domain (CTD) is essential for the transcription activation and pioneering function of EBF1. We identified the specific tyrosine residues in the CTD required for phase separation. We transduced pro-B cells with EBF1 wt, deletion of the entire CTD (dC), or mutation of these tyrosines (YmA). We performed ChIP-seq, ATAC-seq, and RNA-seq to see the effect of disrupting phase separation ability on DNA binding, chromatin opening, and target gene expression. Our findings confirm that phase separation is important for the EBF1 function.

2024-01-18 | GSE227614 | GEO

ATAC-seq of pro-B cells to test the role of phase separation in EBF1 pioneering function

2024-01-18 | GSE227613 | GEO