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Arabidopsis thaliana

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type (Col), hda6, hda5, hda9, fld and fve mutants

PROVIDER: PRJNA314484 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3207070.fastq.gz	Fastqsanger.gz
	SRR3207080.fastq.gz	Fastqsanger.gz
	SRR3207071.fastq.gz	Fastqsanger.gz
	SRR3207072.fastq.gz	Fastqsanger.gz
	SRR3207073.fastq.gz	Fastqsanger.gz

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The Histone Deacetylases HDA6 and HDA9 coordinately regulate valve cell elongation through depressing auxin signaling in Arabidopsis

Project description:Both of Histone Deacetylases HDA6 and HDA9 belong to RPD3/HDA1 class I subfamily, and they have similar protein structure. Loss of function of HDA9 display a blunt silique. Although there is not protein-protein interaction between HDA6 and HDA9, they simultaneously loss function led to “nock-shape” silique that more seriously silique phenotype than hda9. The silique valve cell of hda9 and hda6 hda9 were longer than wild type and hda6. The transcripts level of auxin signaling related genes were mis-regulated in hda9 and hda6 hda9 silique, and GFP signaling derived by auxin response promoter DR5 were weaker in hda9 and hda6 hda9 than wild type and hda6. Thus, our findings reveal that HDA6 and HDA9 coordinately control silique valve cell elongation through affecting auxin signaling related genes expression in silique.

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Project description:Analysis of the transcriptome of dry hda9-1 mutant seeds with those of Col wild-type seeds, using Affymetrix GeneChip Arabidopsis ATH1 Genome Array. The hda9-1 mutant has reduced primary seed dormancy. We used microarrays to dissect which genes are differentially expressed in the hda9-1 mutant to study the mechanisms how HDA9 affects seed dormancy and germination

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2025-05-02 | GSE295963 | GEO

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