Project description:In order to obtain genetically engineering strain of Penicillium oxalicum with high Raw Starch Digesting Glucoamylase production,we deleted PoxCxrC and overexpressed POX_f08097 in Penicillium oxalicum TE4-10 ,and we want to investigating the function of deletion of PoxCxrC and overexpression of POX_f08097 at transcriptional level.
Project description:In order to investigate the regulatory function of POX_a00954 on Raw Starch Digesting Glucoamylase production in Penicillium oxalicum,we deleted POX_a00954 in Penicillium oxalicum Δku70 ,and we want to investigating the function of deletion of POX_a00954 at transcriptional level.
Project description:RNA-seq was used to compare differential gene expressions for Penicillium oxalicum wild type strain (M12) and sporognesis related genes knock out strains.The goals of this study are to construct the sporogenesis regulation pathway of Penicillium oxalicum. Examination of differential gene expressions by digital gene expression tag profiling in Penicillium oxalicum wild type strain and nine mutant strains (flbA knoutout strain, flbB knoutout strain, flbC knoutout strain, flbD knoutout strain, flbE knoutout strain,wetA knoutout strain, abaA knoutout strain,stuA knoutout strain, swi6 knoutout strain)
Project description:In order to studing molecular mechanism of Raw Starch Digesting Glucoamylase production in Penicillium oxalicum ,we deleted POX01907 and POX08340 and analyzed the co-expression network of POX08340 and POX01907 at transcriptional level.
Project description:In order to studing molecular mechanism of Raw Starch Digesting Glucoamylase production in Penicillium oxalicum ,we deleted POX01907 and POX08340 and analyzed the co-expression network of POX08340 and POX01907 at transcriptional level.
Project description:RNA-seq was used to compare differential gene expressions for Penicillium oxalicum wild type strain (M12) and sporognesis related genes knock out strains.The goals of this study are to construct the sporogenesis regulation pathway of Penicillium oxalicum.
Project description:In order to obtain genetically engineering strain of Penicillium oxalicum with high PPDE production,we silenced POc04478 gene after adding 5 μM of copper ions in Penicillium oxalicum strain △PoxKu70 ,and we want to investigating the function of silencing of POc04478 gene after adding 5 μM of copper ions at transcriptional level.
Project description:The goals of this study are to compare differential gene expressions for Penicillium oxalicum wild type strain (WT), and laeA knockout strain (ΔlaeA) in different development phase. The deletion of laeA downregulated genes involved in oxidation- reduction process, alkaloid metabolic process, and transmembrane transport. We find the expression levels of seven secondary metabolism gene clusters (totally 28 clusters) were silenced inΔlaeA. This study provides the information that laeA function are required in conidiation and hydrolase activity of P. oxalicum. Examination of differential gene expressions by digital gene expression tag profiling in Penicillium oxalicum wild type strain and laeA knockout mutant strains in 24h and 60h in modified Czapek culture medium with 2% glucose as carbon resource. qRT–PCR validation was performed using SYBR Green assays.