Project description:The analysis of the Fusarium graminearum transcriptome during symptomless and symptomatic infections of wheat floral tissue at 7 days post infection, to identify the correct spatio-temperol regulation of multiple virulence strategies.
Project description:Fusarium graminearum gene expression profiles were compared during an early infection time course (1, 2, and 4d post-inoculation) of three monocot hosts (wheat heads, barley heads, and maize developing kernels).
Project description:We report the transcriptome profile of different cultivars of Fusarium graminearum-infected wheat grains, aiming to search for some different expression genes and pathways to reveal the difference between wheat cultivars.
Project description:The plant pathogenic fungus Fusarium graminearum (Fgr) creates economic and health risks in cereals agriculture. Fgr causes head blight (or scab) of wheat and stalk rot of corn, reducing yield, degrading grain quality and polluting downstream food products with mycotoxins. Fungal plant pathogens must secrete proteases to access nutrition and to breakdown the structural protein component of the plant cell wall. Research into the proteolytic activity of Fgr is hindered by the complex nature of the suite of proteases secreted. We used a systems biology approach comprising genome analysis, transcriptomics and label-free quantitative proteomics to characterise the peptidases deployed by Fgr during growth. A combined analysis of published microarray transcriptome datasets revealed seven transcriptional groupings of peptidases based on in vitro growth, in planta growth, and sporulation behaviours. An orbitrap MS/MS proteomics technique defined the extracellular proteases secreted by Fusarium graminearum.
Project description:Fusarium cereal pathogens are known to produce a diverse array of mycotoxins and other secondary metabolites which may act together to cause plant disease and adverse effects on human and animal health. To study the impact of Fusarium interactions, we chose F. graminearum and F. avenaceum, which are known to co-contaminate Canadian durum wheat samples resulting in the deposition of trichothecenes, enniatins, moniliformin, and other bioactive compounds. We are studying the interaction of a F. graminearum (3-ADON-producer) and a F. avenaceum strain (both isolated from the same wheat sample) in durum wheat spikes. To study the impact of F. avenaceum secondary metabolites on F. graminearum, we conducted transcriptomic profiling of F. graminearum grown in the presence of F. avenaceum culture filtrate extracts in vitro. A F. graminearum ABC transporter gene was strongly induced in vitro upon exposure to F. avenaceum culture filtrate.
Project description:Fusarium head blight (FHB) is a major disease of cereal crops caused by the fungus Fusarium graminearum (Fg). FHB affects the flowering heads (or spikes). This study compare the gene expression profile in wheat spikelets from the resistant spring wheat cultivar NuyBay inoculated with water (H2O) or Fg.
Project description:Fusarium head blight (FHB) is a major disease of cereal crops caused by the fungus Fusarium graminearum (Fg). FHB affects the flowering heads (or spikes). This study compare the gene expression profile in wheat spikelets from the very susceptible spring wheat cultivar Roblin inoculated with water (H2O) or Fg.
Project description:Fusarium head blight (FHB) is a major disease of cereal crops caused by the fungus Fusarium graminearum (Fg). FHB affects the flowering heads (or spikes). This study compare the gene expression profile in wheat spikelets from the resistant winter wheat cultivar Dream inoculated with water (H2O) or Fg.
Project description:Fusarium head blight (FHB) is a major disease of cereal crops caused by the fungus Fusarium graminearum (Fg). FHB affects the flowering heads (or spikes). This study compare the gene expression profile in wheat spikelets from the resistant spring wheat cultivar Wuhan1 inoculated with water (H2O) or Fg.
