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Yarrowia lipolytica

ABSTRACT: Medium pH influences citric acid and lipid production in oleaginous yeast Yarrowia lipolytica

PROVIDER: PRJNA319797 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3457620.fastq.gz	Fastqsanger.gz
	SRR3457621.fastq.gz	Fastqsanger.gz
	SRR3457622.fastq.gz	Fastqsanger.gz
	SRR3457623.fastq.gz	Fastqsanger.gz
	SRR3457624.fastq.gz	Fastqsanger.gz

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Similar Datasets

Medium pH influences citric acid and lipid production in oleaginous yeast Yarrowia lipolytica

Project description:The goal of this study is to find out how Yarrowia lipolytica W29’s transcriptome changes in response to different pHs in certain medium, where the production of citric acid and lipid vary significantly at different pHs. The changes in transcriptome will give us insight about the mechanism of citric acid and/or lipid production are regulated. We harvested Y. lipolytica cells from bioreactor cultures from three different pHs: 2.0, 4.0 and 6.0 in triplicates. We sequenced the mRNA by Illumina HiSeq 2500 and generated an average of 20 million reads for each sample. We did not find distinct gene expression changes for the enzymes right downstream the intersection where citrate is cleaved into acetyl-CoA. Instead, from gene ontology enrichment analysis, we observed proteins with transport activities were overrepresented in gene groups that were up-regulated higher pH conditions, where citric acid secretion is favored.

2019-04-26 | GSE80717 | GEO

Yarrowia lipolytica fed-batch biolipid production.

2011-05-15 | GSE29046 | GEO

Yarrowia lipolytica biolipid production during D-stat setup. [Part 2]

Project description:Second part of the time-course transcriptomic profilling of the oleaginous yeast Yarrowia lipolytica, obtained during a controlled decellerostat (D-stat) setup. A nitrogen limitation was applied during the course of the D-stat to initiate and control de novo biolipid synthesis. Yarrowia lipolytica Agilent microarray, one-color staining. Each time sample was spotted in triplicate (except for sample 26, for which a technical replicate of replicate r1 was performed).

2015-01-01 | E-GEOD-35446 | biostudies-arrayexpress

Yarrowia lipolytica biolipid production during D-stat setup. [Part 1]

Project description:First part of the time-course transcriptomic profilling of the oleaginous yeast Yarrowia lipolytica, obtained during a controlled decellerostat (D-stat) setup. A nitrogen limitation was applied during the course of the D-stat to initiate and control de novo biolipid synthesis. Yarrowia lipolytica Agilent microarray, one-color staining. Each time sample was spotted in triplicate. A reference condition was spotted in quadruplicate, based on a RNA pool from three samples obtained during the biomass production phase.

2015-01-01 | E-GEOD-35445 | biostudies-arrayexpress

Yarrowia lipolytica biolipid production during D-stat setup. [Part 1]

2015-01-01 | GSE35445 | GEO

Yarrowia lipolytica biolipid production during D-stat setup. [Part 2]

2015-01-01 | GSE35446 | GEO

Absolute expression level of Yarrowia lipolytica genes

Project description:Total RNA versus genomic DNA hybridization on custom arrays designed for all Yarrowia lipolytica genes Total RNA was collected in mid-log phase from Yarrowia lipolytica cells grown in rich medium (abbreviated CM, in house recipe). RNA was then converted to cDNA, Cy3-labeled and hybridized competitively against Cy5 labeled genomic DNA from Yarrowia lipolytica.

2010-07-06 | E-GEOD-22192 | biostudies-arrayexpress

Yarrowia lipolytica fed-batch biolipid production.

Project description:Time-course transcriptomic profilling of the oleaginous yeast Yarrowia lipolytica, during a controlled fed-batch. A nitrogen limitation was applied during the course of the fed-batch to initiate de novo biolipid synthesis. Dual staining, one replicate per slide. On each slide, a time point sample and a reference sample are spotted. Reference samples were obtained by pooling the RNAs from the time-course samples.

2011-05-15 | E-GEOD-29046 | biostudies-arrayexpress

Functional genomics for the oleaginous yeast Yarrowia lipolytica

Project description:Oleaginous yeasts are valuable systems for biosustainable production of hydrocarbon-based chemicals. Yarrowia lipolytica is one of the best characterized of these yeast with respect to genome annotation and flux analysis of metabolic processes. Nonetheless, progress is hampered by a dearth of genomewide tools enabling functional genomics. The Hermes DNA transposon was expressed to achieve saturation mutagenesis of the Y. lipolytica genome. Over 535 thousand independent insertions were identified by next-generation sequencing. Poisson analysis of insertion density classified ~22% of genes as essential. As expected, most essential genes not only have homologues in Saccharomyces cerevisiae and Schizosaccharomyces pombe, but the majority of those are also essential. As an obligate aerobe, Y. lipolytica has significantly more respiration - related genes that are classified as essential than do S. cerevisiae and S. pombe. The findings provide insights into biosynthetic pathways, compartmentalization of enzymes, and distinct functions of paralogs. Contributions of nonessential genes to fitness were determined in log growth cultures with glucose and glycerol carbon sources. Fluorescence-activated cell sorting identified mutants in which lipid accumulation is increased. Biological contributions of genes to growth were used to evaluate two recent genome-scale models Y. lipolytica metabolism. This study is the first functional genomic analysis of an oleaginous yeast and provides an important resource for modeling and bioengineering of Y. lipolytica.

2018-12-06 | GSE90993 | GEO

Expression analysis of Yarrowia lipolytica Y4184 snf1 mutant

Project description:Investigation of whole genome gene expression level changes in a Yarrowia lipolytica Y4184 snf1 mutant, compared to the Y4184U+. The Y4184 is an engineered strain to produce eicosapentaenoic acid (EPA) via expression of a Δ9 elongase/Δ8 desaturase pathway, and is derived from Yarrowia lipolytica ATCC#20362.

2013-09-25 | GSE45611 | GEO

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