Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Regulation of gene transcription and transcription factor chromatin binding by PARP-1 in mouse embryonic stem cells

ABSTRACT: Regulation of gene transcription and transcription factor chromatin binding by PARP-1 in mouse embryonic stem cells

PROVIDER: PRJNA320770 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR2673300.fastq.gz	Fastqsanger.gz
	SRR2673310.fastq.gz	Fastqsanger.gz
	SRR2673301.fastq.gz	Fastqsanger.gz
	SRR2673311.fastq.gz	Fastqsanger.gz
	SRR2673192.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

Nucleosome binding by the pioneer transcription factor OCT4

Project description:The nucleosome is a fundamental unit of chromatin in eukaryotes, and generally prevents the binding of transcription factors to genomic DNA. Pioneer transcription factors overcome the nucleosome barrier, and bind their target DNA sequences in chromatin. OCT4 is a representative pioneer transcription factor that plays a role in stem cell pluripotency. In the present study, we biochemically analyzed the nucleosome binding by OCT4. Crosslinking mass spectrometry showed that OCT4 binds the nucleosome.

2020-05-13 | PXD019160 | JPOST Repository

Genome-wide binding of transcription factor IRF3

Project description:Genome-wide binding of transcription factor IRF3

| PRJNA686372 | ENA

Mapping of transcription factor binding sites in mouse embryonic stem cells

Project description:Transcription factors and their specific interactions with targets are crucial in specifying gene expression programs. To gain insights into the transcriptional regulatory networks in embryonic stem cells, we use chromatin immunoprecipitation coupled to ultra-high-throughput DNA sequencing (ChIP-seq) to map the locations of thirteen sequence specific transcription factors (Nanog, Oct4, STAT3, Smad1, Sox2, Zfx, c-Myc, n-Myc, Klf4, Esrrb, Tcfcp2l1, E2f1 and CTCF) and two transcription regulators (p300 and Suz12). These factors are known to play different roles in ES cell biology as components of the LIF and BMP signaling pathways, self-renewal regulators and key reprogramming factors. Our study provides insights into the integration of the signaling pathways to the ES cell-specific transcription circuitries. Intriguingly, we find specific genomic regions extensively targeted by different transcription factors. Collectively, the comprehensive mapping of transcription factor binding sites identifies important features of the transcriptional regulatory networks that define ES cell identity. Keywords: Transcription factor binding sites in undifferentiated mouse embryonic stem cells

2008-06-12 | GSE11431 | GEO

Transcription Factor binding evolution in five mouse species

Project description:Transcription Factor binding evolution in five mouse species

| PRJEB1247 | ENA

Transcription factor binding in early pancreatic progenitors (PP1)

Project description:Transcription factor binding in early pancreatic progenitors (PP1)

| PRJNA1137383 | ENA

DNA bendability regulates transcription factor binding to nucleosomes

Project description:DNA bendability regulates transcription factor binding to nucleosomes

| PRJNA1244853 | ENA

Differential binding of PARP-1 domains

Project description:Differential binding of PARP-1 domains

| PRJNA923684 | ENA

Parp-1 wildtype and deficient mouse embryonic stem cells

Project description:Genome-wide methylation analysis was performed by methylated DNA immunoprecipitation (MeDIP)-CpG island (CGI) microarray analysis to identify the CpG methylation levels of Parp-1 wildtype, Parp-1 deficient or Parp inhibitor treated mouse embryonic stem cells.

2014-12-31 | GSE37651 | GEO

The role of PARP-1 in estrogen-dependent transcription [ChIP-seq]

Project description:Poly (ADP-ribose) polymerase-1 (PARP-1), a multifunctional chromatin-modulating protein, has gained considerable attention as a target for therapeutic inhibitors in breast cancers. Accumulating evidence suggests a pathological role for PARP-1 in breast cancer through its effects on the transcription of tumor-related genes. Here we report the role of PARP-1 in estrogen-dependent transcription in estrogen receptor alpha (ERα)-positive breast cancers. Global nuclear run-on and sequencing (GRO-seq) analyses suggest that PARP-1 controls the expression of estrogen-regulated genes in ER-positive (ER+) MCF-7 breast cancer cells. Further, ChIP-seq analyses revealed that PARP-1 directly regulates the ligand-dependent binding of ERa and FoxA1 to a subset of its genomic binding sites. Finally, we uncovered that the expression levels of the PARP-1 and estrogen coregulated gene set are enriched in luminal molecular-subtype of breast tumors and high PARP-1 expression in ER+ cases correlates with poor survival. Additionally, treatment with PARP-1 selective inhibitors showed attenuated estrogen-dependent growth of ER+ breast cancer cells. Taken together, the current study suggests that PARP-1 regulates critical molecular pathways that underlie proliferation of ER+ breast cancer cells.

2021-10-13 | GSE166168 | GEO

Combinatorial transcription factor binding evolution in five placental mammals

Project description:Combinatorial transcription factor binding evolution in five placental mammals

| PRJEB1571 | ENA