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Brassica napus

ABSTRACT: RNA profiling of sclerotinia-infected leaves of Brassica napus reveals cross talk between redox and hormone pathways

PROVIDER: PRJNA321917 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3537150.fastq.gz	Fastqsanger.gz
	SRR3537160.fastq.gz	Fastqsanger.gz
	SRR3537151.fastq.gz	Fastqsanger.gz
	SRR3537161.fastq.gz	Fastqsanger.gz
	SRR3537152.fastq.gz	Fastqsanger.gz

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Transcription profiling of Brassica napus leaves inoculated by Sclerotinia sclerotiorum and harvested after 12, 24 and 48 h

Project description:Brassica napus leaves(18 days old) were inoculated by Sclerotinia sclerotiorum with leaves harvested after 12, 24 and 48 h. Arabidopsis thaliana full-genome 70mer microarray representing at least 23,686 genes were used.

2007-10-01 | E-MEXP-1031 | biostudies-arrayexpress

BnaA07.WRKY40 transcription factor confers enhanced defensive response to sclerotinia sclerotiorum in Brassica napus

Project description:Oilseed rape (Brassica napus, B. napus) is one of the most important oil crops globally, contributing significantly to the world's supply of vegetable oil. However, its production is severely threatened by Sclerotinia stem rot, a disease caused by the broad-host-range fungus Sclerotinia sclerotiorum (Lib.) de Bary (S. sclerotiorum). We have investigated the gene expression of J9712 and W40-OE2 during different time periods of Sclerotinia sclerotiorum infection through RNA-Seq analysis.

2024-12-19 | GSE284750 | GEO

RNA profiling of sclerotinia-infected leaves of Brassica napus reveals cross talk between redox and hormone pathways

Project description:Global transcriptome profiling of suceptible and tolerant lines of Brassica napus infected with Sclerotinia sclerotiorum using a petal inoculation method that mimics field conditions.

2017-08-01 | GSE81545 | GEO

Sequential expression of Sclerotinia sclerotiorum pathogenicity factors during infection of Brassica napus as revealed by RNA-Seq analysis

Project description:RNA-seq of Sclerotinia sclerotiorum-infected B. napus leaves during a timecourse after inoculation. This is a time course experiment. Sclerotinia hyphae were place on detached B. napus leaves, then the combined tissues were harvested at 1, 3, 6, 12, 24, and 48 hours post-innoculation. 3 biological replicates were collected for each time point, and sclerotinia from liquid medium was used as a control.

2016-07-01 | E-GEOD-83935 | biostudies-arrayexpress

Sequential expression of Sclerotinia sclerotiorum pathogenicity factors during infection of Brassica napus as revealed by RNA-Seq analysis

Project description:RNA-seq of Sclerotinia sclerotiorum-infected B. napus leaves during a timecourse after inoculation.

2016-07-01 | GSE83935 | GEO

Sclerotinia infected vs Mock infected controls in B. napus Westar and Zhong You 821

Project description:This SuperSeries is composed of the following subset Series: GSE13254: Sclerotinia infected vs Mock infected controls in B. napus (Westar) GSE13256: Zhong You 821 Sclerotinia infected vs Mock infected controls in B. napus (Zhong You 821) The fungal pathogen Sclerotinia sclerotiorum infects a broad range of dicotyledonous plant species and is the causative agent of stem rot in Brassica napus. To elucidate the mechanisms underlying the defense response, we studied the patterns of gene expression in a partially resistant variety of ZhongYou 821 (ZY821) and a susceptible line from Westar over five time points, 6, 12, 24, 48, and 72 hours post-inoculation (hpi) using a B. napus oligonucleotide microarray. For each cultivar, a two-dye experiment was run comparing infected to mock-infected stem tissue. For each time point, 6 microarray slides were done (3 biological replicates, with a dye swap for each biological replicate). Refer to individual Series

2009-01-31 | E-GEOD-13262 | biostudies-arrayexpress

Subgenome-dominant expression and alternative splicing in response to pathogen stress in polyploid Brassica napus and progenitors

Project description:Polyploidy has played an extensive role in the evolution of flowering plants. Allopolyploids, with subgenomes containing duplicated gene pairs called homeologs, can show rapid transcriptome changes including novel alternative splicing (AS) patterns. The extent to which abiotic stress modulates AS of homeologs is a nascent topic in polyploidy research. We subjected both natural and resynthesized lines of polyploid Brassica napus, along with the progenitors B. rapa and B. oleracea, to infection with the fungal pathogen Sclerotinia sclerotiorum. RNA-seq analyses revealed widespread divergence between polyploid subgenomes in both gene expression and AS patterns. Resynthesized B. napus displayed significantly more A and C subgenome biased homeologs under pathogen infection than during uninfected growth. Differential AS (DAS) in response to infection was highest in natural B. napus (12,709 DAS events) and lower in resynthesized Brassica napus (8,863 DAS events). Natural B. napus had more up-regulated events and fewer down-regulated events. There was a global expression bias towards the B. oleracea-derived (C) subgenome in both resynthesized and natural B. napus, enhanced by widespread non-parental downregulation of the B. rapa-derived (A) homeolog. In the resynthesized B. napus specifically, this resulted a disproportionate C subgenome contribution to pathogen defense response, characterized by biases in both transcript expression levels and the proportion of induced genes. Our results elucidate the complex ways in which Sclerotinia infection affects expression and AS of homeologous genes in natural and resynthesized B. napus, and indicate that abiotic stress can influence the evolution of homeologous genes in polyploids.

2023-02-04 | GSE201225 | GEO

Transcriptomic and metabolomic analysis reveal that fullerol improves drought tolerance in Brassica napus L.

Project description:The fullerenes, a kind of carbon nanoparticles, have potential for enhanced stress tolerance in plants. While the positive effects of polyhydroxy fullerene—fullerol on plants in response to drought at the physiological level have been documented, the molecular mechanism in Brassica napus are not entirely understood. In this study, exogenous fullerol was applied to the leaves of B. napus seedlings given drought. The leaves of B. napus seedlings in each treatment (sufficient water condition, drought, and drought combined with fullerol) were used to conduct the molecular mechanism using transcriptomic analysis.

2022-12-14 | GSE216368 | GEO

RNAseq of Brassica napus seed

Project description:mRNA expression profiling of the embryo, endosperm (micropylar, peripheral, chalazal), and seed coat (outer, inner, chalazal, chalazal proliferating tissue) of the developing Brassica napus seed. Tissues were isolated using laser microdissection (LMD) from Brassica napus seeds at the globular, heart, and mature green stages of seed development.

2024-07-15 | GSE271809 | GEO

Sclerotinia infected vs Mock infected controls in B. napus Westar and Zhong You 821

Project description:The fungal pathogen Sclerotinia sclerotiorum infects a broad range of dicotyledonous plant species and is the causative agent of stem rot in Brassica napus. To elucidate the mechanisms underlying the defense response, we studied the patterns of gene expression in a partially resistant variety of ZhongYou 821 (ZY821) and a susceptible line from Westar over five time points, 6, 12, 24, 48, and 72 hours post-inoculation (hpi) using a B. napus oligonucleotide microarray. For each cultivar, a two-dye experiment was run comparing infected to mock-infected stem tissue. For each time point, 6 microarray slides were done (3 biological replicates, with a dye swap for each biological replicate). This SuperSeries is composed of the SubSeries listed below.

2009-02-01 | GSE13262 | GEO

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