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Brassica napus

ABSTRACT: Tight Regulation of the Interaction between Brassica napus and Sclerotinia sclerotiorum at the MicroRNA Level [HTS]

PROVIDER: PRJNA322006 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3539824.fastq.gz	Fastqsanger.gz
	SRR3539825.fastq.gz	Fastqsanger.gz
	SRR3539827.fastq.gz	Fastqsanger.gz

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BnaA07.WRKY40 transcription factor confers enhanced defensive response to sclerotinia sclerotiorum in Brassica napus

Project description:Oilseed rape (Brassica napus, B. napus) is one of the most important oil crops globally, contributing significantly to the world's supply of vegetable oil. However, its production is severely threatened by Sclerotinia stem rot, a disease caused by the broad-host-range fungus Sclerotinia sclerotiorum (Lib.) de Bary (S. sclerotiorum). We have investigated the gene expression of J9712 and W40-OE2 during different time periods of Sclerotinia sclerotiorum infection through RNA-Seq analysis.

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RNA profiling of sclerotinia-infected leaves of Brassica napus reveals cross talk between redox and hormone pathways

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Transcription profiling of Brassica napus leaves inoculated by Sclerotinia sclerotiorum and harvested after 12, 24 and 48 h

Project description:Brassica napus leaves(18 days old) were inoculated by Sclerotinia sclerotiorum with leaves harvested after 12, 24 and 48 h. Arabidopsis thaliana full-genome 70mer microarray representing at least 23,686 genes were used.

2007-10-01 | E-MEXP-1031 | biostudies-arrayexpress

Brassica napus

Project description:Tight Regulation of the Interaction between Brassica napus and Sclerotinia sclerotiorum at the MicroRNA Level [array]

| PRJNA322007 | ENA

Host Induced Gene Silencing of the Sclerotinia sclerotiorum ABHYDROLASE-3 gene reduces disease severity in Brassica napus

Project description:Sclerotinia sclerotiorum is a pathogenic fungus that infects hundreds of crop species, causing extensive yield loss every year. Chemical fungicides are used to control this phytopathogen, but with concerns about increasing resistance and impacts on non-target species, there is a need to develop alternative control measures. In the present study, we engineered Brassica napus to constitutively express a hairpin (hp)RNA molecule to silence ABHYRDOLASE-3 in S. sclerotiorum. We demonstrate the potential for Host Induced Gene Silencing (HIGS) to protect B. napus from S. sclerotiorum using leaf, stem and whole plant infection assays. The interaction between the transgenic host plant and invading pathogen was further characterized at the molecular level using dual-RNA sequencing and at the anatomical level through microscopy to understand the processes and possible mechanisms leading to increased tolerance to this damaging necrotroph. We observed significant shifts in the expression of genes relating to plant defense as well as cellular differences in the form of structural barriers around the site of infection in the HIGS-protected plants. Our results provide proof-of-concept that HIGS is an effective means of limiting damage caused by S. sclerotiorum to the plant and demonstrates the utility of this biotechnology in the development of resistance against fungal pathogens.

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Host Induced Gene Silencing of the Sclerotinia sclerotiorum ABHYDROLASE-3 gene reduces disease severity in Brassica napus [small RNA]

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Sequential expression of Sclerotinia sclerotiorum pathogenicity factors during infection of Brassica napus as revealed by RNA-Seq analysis

Project description:RNA-seq of Sclerotinia sclerotiorum-infected B. napus leaves during a timecourse after inoculation.

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Tight Regulation of the Interaction between Brassica napus and Sclerotinia sclerotiorum at the MicroRNA Level [array]

Project description:MicroRNAs are multifunctional non-coding short nucleotide molecules. Nevertheless, the role of miRNAs in the interactions between plants and necrotrophic pathogens is largely unknown. Here, we report the identification of the miRNA repertoire of the economically important oil crop oilseed rape (Brassica napus) and those involved in interacting with its most devastating necrotrophic pathogen Sclerotinia sclerotiorum. We identified 280 B. napus miRNA candidates, including 53 novel candidates and 227 canonical members or variants of known miRNA families, by high-throughput deep sequencing of small RNAs from both normal and S. sclerotiorum-inoculated leaves. Target genes of 15 novel candidates and 222 known miRNAs were further identified by sequencing of degradomes from the two types of samples. MicroRNA microarray analysis revealed that 68 miRNAs were differentially expressed between S. sclerotiorum-inoculated and uninoculated leaves. A set of these miRNAs target genes involved in plant defense to S. sclerotiorum and/or other pathogens such as NBS-LRR R genes and nitric oxygen and reactive oxygen species related genes. Additionally, three miRNAs target AGO1 and AGO2, key components of post-transcriptional gene silencing (PTGS). Expression of several viral PTGS suppressors reduced resistance to S. sclerotiorum. Arabidopsis mutants of AGO1 and AGO2 exhibited reduced resistance while transgenic lines over-expressing AGO1 displayed increased resistance to S. sclerotiorum in an AGO1 expression level-dependent manner. Moreover, transient over-expression of miRNAs targeting AGO1 and AGO2 decreased resistance to S. sclerotiorum in oilseed rape. Our results demonstrate that the interactions between B. napus and S. sclerotiorum are tightly regulated at miRNA level and probably involve PTGS.

2017-05-16 | GSE81560 | GEO

Tight Regulation of the Interaction between Brassica napus and Sclerotinia sclerotiorum at the MicroRNA Level [HTS]

2017-05-16 | GSE81561 | GEO

Sequential expression of Sclerotinia sclerotiorum pathogenicity factors during infection of Brassica napus as revealed by RNA-Seq analysis

Project description:RNA-seq of Sclerotinia sclerotiorum-infected B. napus leaves during a timecourse after inoculation. This is a time course experiment. Sclerotinia hyphae were place on detached B. napus leaves, then the combined tissues were harvested at 1, 3, 6, 12, 24, and 48 hours post-innoculation. 3 biological replicates were collected for each time point, and sclerotinia from liquid medium was used as a control.

2016-07-01 | E-GEOD-83935 | biostudies-arrayexpress

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