Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mus musculus

ABSTRACT: Autophagy maintains metabolism and functional activity of a subset of aged hematopoietic stem cells [RRBS]

PROVIDER: PRJNA322387 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR3566380.fastq.gz	Fastqsanger.gz
	SRR3566381.fastq.gz	Fastqsanger.gz
	SRR3566382.fastq.gz	Fastqsanger.gz
	SRR3566383.fastq.gz	Fastqsanger.gz
	SRR3566376.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Autophagy maintains metabolism and functional activity of a subset of aged hematopoietic stem cells

Project description:Autophagy maintains metabolism and functional activity of a subset of aged hematopoietic stem cells

| PRJNA322385 | ENA

Mus musculus

Project description:Autophagy maintains metabolism and functional activity of a subset of aged hematopoietic stem cells [ERRBS]

| PRJNA351841 | ENA

Mus musculus

Project description:Autophagy maintains metabolism and functional activity of a subset of aged hematopoietic stem cells [gene expression]

| PRJNA322388 | ENA

Project description:Rora Activity Regulates Aged Hematopoietic Stem Cell Phenomes

| PRJNA703313 | ENA

Project description:Rora Activity Regulates Aged Hematopoietic Stem Cell Phenomes

| PRJNA706345 | ENA

Chaperone-mediated autophagy sustains muscle stem cell regenerative function

Project description:Proteostasis supports stemness and its loss correlates with the functional decline of diverse stem cell types. Here we identify that chaperone-mediated autophagy (CMA), a selective autophagy pathway, is necessary for the regenerative capacity of muscle stem cells (MuSCs) throughout life. We show that CMA is active in MuSCs, while genetic loss of CMA in MuSCs or failure of CMA in normally aged cells causes a proliferative impairment, resulting in defective skeletal muscle regeneration. Reactivation of CMA in old MuSCs boosts their proliferative capacity and improves their regenerative ability. Using comparative proteomics to identify CMA substrates, we uncovered actin cytoskeleton and glycolytic metabolism as key processes altered in aged mice and human MuSCs. Our findings reveal CMA to be a decisive stem-cell-fate regulator, with implications for fostering muscle regeneration in old age.

2025-10-17 | PXD059966 | Pride

Chaperone-mediated autophagy sustains muscle stem cell regenerative functions but declines with age

2025-10-15 | GSE286877 | GEO

Sex hormones control muscle stem cells

Project description:Quiescent adult muscle stem cells (MuSCs) regenerate skeletal muscle upon injury throughout life. However, aged skeletal muscles fail to maintain stem cell quiescence, leading to declines in MuSC number and functionality. Although autophagy plays an important role in the maintenance of MuSC quiescence, how quiescent MuSCs and their autophagy levels are maintained throughout life is largely unknown. The current study reveals how GnRH, a hypothalamic hormone, maintains the quiescence of adult MuSCs by preventing the onset of senescence and how the decline of sex steroids in organismal ageing is implicated in MuSC ageing.

2019-10-05 | GSE121016 | GEO

Chaperone-mediated autophagy sustains hematopoietic stem cell function

Project description:Activation of mostly quiescent hematopoietic stem cells (HSC) is a prerequisite for life-long blood production1, 2. This process requires major molecular adaptations to meet the regulatory and metabolic requirements for cell division3-8. The mechanisms governing cellular reprograming upon stem cell activation and their subsequent return to quiescence are still not fully characterized. Here, we describe a role for chaperone-mediated autophagy (CMA)9, a selective form of lysosomal protein degradation, in sustaining adult HSC function. CMA is required for stem cell protein quality control and upregulation of fatty acid metabolism upon HSC activation. We identify that CMA activity decreases with age in HSC and show that genetic or pharmacological activation of CMA can restore functionality of old HSC. Together, our findings provide mechanistic insights into a new role for CMA in sustaining quality control, appropriate energetics and overall long-term hematopoietic stem cell function. Our work supports that CMA may be a promising therapeutic target to enhance hematopoietic stem cell function in conditions such as aging or stem cell transplantation.

2021-03-05 | PXD022196 | Pride

Autophagy maintains intestinal stem cell integrity

Project description:Autophagy maintains intestinal stem cell integrity

| PRJNA564106 | ENA