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BRD4 localization to lineage-specific enhancers is associated with a distinct transcription factor repertoire

ABSTRACT: BRD4 localization to lineage-specific enhancers is associated with a distinct transcription factor repertoire

PROVIDER: PRJNA324572 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3631720.fastq.gz	Fastqsanger.gz
	SRR3631730.fastq.gz	Fastqsanger.gz
	SRR3632000.fastq.gz	Fastqsanger.gz
	SRR3632010.fastq.gz	Fastqsanger.gz
	SRR3632020.fastq.gz	Fastqsanger.gz

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BRD4 localization to lineage-specific enhancers is associated with a distinct transcription factor repertoire [ChIP-Seq]

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Project description:Proper temporal epigenetic regulation of gene expression is essential for cell fate determination and tissue development. The Bromodomain-containing Protein-4 (BRD4) was previously shown to control the transcription of defined subsets of genes in various cell systems. In this study we examined the role of BRD4 in promoting lineage-specific gene expression and show that BRD4 is essential for osteoblast differentiation. Genome-wide analyses demonstrate that BRD4 is recruited to the transcriptional start site of differentiation-induced genes. Unexpectedly, while promoter-proximal BRD4 occupancy correlated with gene expression, genes which displayed moderate expression and promoter-proximal BRD4 occupancy were most highly regulated and sensitive to BRD4 inhibition. Therefore, we examined distal BRD4 occupancy and uncovered a specific co-localization of BRD4 with the transcription factors C/EBPb, TEAD1, FOSL2 and JUND at putative osteoblast-specific enhancers. These findings reveal the intricacies of lineage specification and provide new insight into the context-dependent functions of BRD4.

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BRD4 is necessary for epidermal differentiation

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BRD4 localization to lineage-specific enhancers is associated with a distinct transcription factor repertoire

Project description:This SuperSeries is composed of the SubSeries listed below.

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