Project description:LncRNA and mRNA microarrays were performed to identify differentially expressed lncRNAs and mRNAs in fibroblast-like synoviocytes from rheumatoid arthritis patients compared with fibroblast-like synoviocytes from trauma patients. Fibroblast-like synoviocytes were isolated from synovial tissues. LncRNA and mRNA microarrays were performed using fibroblast-like synoviocytes at passage 3.
Project description:Fibroblast-like synoviocytes (FLSs) are critical for synovial aggressiveness and joint destruction in rheumatoid arthritis (RA).The role and expression patterns of long noncoding RNAs (lncRNAs) in RA are largely unknown. We performed lncRNA and mRNA microarrays to identify differentially expressed lncRNAs and mRNAs in fibroblast-like synoviocytes from rheumatoid arthritis patients compared with fibroblast-like synoviocytes from trauma patients.
Project description:LncRNA and mRNA microarrays were performed to identify differentially expressed lncRNAs and mRNAs in fibroblast-like synoviocytes from rheumatoid arthritis patients compared with fibroblast-like synoviocytes from trauma patients.
Project description:To characterize transcriptome changes upon ATF6α knockdown by the siRNA in rheumatoid arthritis fibroblast-like synoviocytes (RA FLSs)
Project description:To explore the downstream target of MSMP, RNA-sequencing (RNA-seq) was used to display gene expression profiles in fibroblast-like synoviocytes (FLSs) from 3 patients with rheumatoid arthritis (RA) between control siRNA (siC) group and siMSMP-2 group (with the highest interference efficiency for MSMP). The heatmap and volcano diagram showed a total of 216 differentially expressed genes with 77 upregulated genes and 139 downregulated genes (Fold change≥1.5，p<0.05)
