Arabidopsis thaliana cultivar:columbia
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA325158 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR3657710_1.fastq.gz | Fastqsanger.gz | |||
| SRR3657710_2.fastq.gz | Fastqsanger.gz | |||
| SRR3657709_1.fastq.gz | Fastqsanger.gz | |||
| SRR3657709_2.fastq.gz | Fastqsanger.gz | |||
| SRR4015220_1.fastq.gz | Fastqsanger.gz |
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Project description:Water use efficiency has long been considered as an important target for the breeding of improved plant performance under drought. Minimizing leaf transpirational water loss via reduction of stomatal water conductance plays a key contributory role in drought resistance. In this study, we employed both guard cell (GC) targeted and constitutive ectopic overexpression of the Target of Rapamycin (TOR) kinase, a master regulator of multiple signaling networks in transgenic Arabidopsis thaliana, to investigate the impact of these expressed AtTOR transgenes in response to drought and water use efficiency. We performed genome-wide transcriptome analysis employing RNA-seq on the three Arabidopsis genotypes grown on the three water treatments, and further analysis will be used to elucidate the potential mechanism(s) contributing to differences in leaf stomatal physiology between WT and transgenic lines.
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Project description:Plants engineered for abiotic stress tolerance may soon be commercialized. The engineering of these plants typically involves the manipulation of complex multigene networks and may therefore have a greater potential to introduce pleiotropic effects than the simple monogenic traits that currently dominate the plant biotechnology market. Drought- tolerant Arabidopsis thaliana were engineered through overexpression of the transcription factor ABF3 in order to investigate unintended pleiotropic effects. In order to eliminate position effects, the Cre/lox recombination system was used to create control plant lines that contain identical T-DNA insertion sites but with the ABF3 transgene excised. This additionally allowed us to determine if Cre recombinase can cause unintended effects that impact the transcriptome. Microarray analysis of control plant lines that underwent Cre-mediated excision of the ABF3 transgene revealed only two genes that were differentially expressed in more than one plant line, suggesting that the impact of Cre recombinase on the transcriptome was minimal. In the absence of drought stress, overexpression of ABF3 had no effect on the transcriptome, but following drought stress, differences were observed in the gene expression patterns of plants overexpressing ABF3 relative to control plants. Examination of the functional distribution of the differentially expressed genes revealed strong similarity indicating that unintended pathways were not activated. In response to drought stress, overexpression of ABF3 results in a reprogramming of the drought response, which is characterized by changes in the timing or strength of expression of some drought response genes, without activating any unexpected gene networks. These results illustrate that important gene networks are highly regulated in Arabidopsis and that engineering stress tolerance may not necessarily cause extensive changes to the transcriptome.
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Project description:The Overexpression of AtCAMTA5 leads to higher plant weight and seed weight as compare to Col-0. Under drought condition overexpression lines had reduce water loss,high water use efficiency decline in photosynthesis contributes to 95% survivability wherase Col-0 and camta5 showed enhance sensitivity.Microarray analysis revealed that AtCAMTA5 regulates genes enriched with CAMTA recognition motif like ANAC19, SPL16, aminotransferase, chitinase, MAPK7, SnRK2.2, E3-ligase, RUBISCO, PSI and PSII, etc which were involved in stress response, development, protein modification and photosynthesis. We used affymatrix expression analysis to validate role of CAMTA5 under drought stress along with physiological and biochemical assay.
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