Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Tetrahymena thermophila

ABSTRACT: Chromatin Immunoprecipitation sequencing of heterochromation associated proteins in Tetrahymena

PROVIDER: PRJNA325440 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR3659030.fastq.gz	Fastqsanger.gz
	SRR3659031.fastq.gz	Fastqsanger.gz
	SRR3659032.fastq.gz	Fastqsanger.gz
	SRR3659033.fastq.gz	Fastqsanger.gz
	SRR3659034.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Tetrahymena thermophila

Project description:Chromatin immunoprecipitation sequencing of heterochromatin-associated proteins in Tetrahymena

| PRJNA287575 | ENA

Tetrahymena thermophila

Project description:Tetrahymena thermophila Twi8p-associated small RNAs

| PRJNA306046 | ENA

Tetrahymena thermophila

Project description:Tetrahymena thermophila

| PRJNA11958 | ENA

Tetrahymena thermophila

Project description:Nucleosome mapping of Tetrahymena thermophila chromatin in vivo and in vitro

| PRJNA269988 | ENA

Tetrahymena thermophila

Project description:Tetrahymena thermophila 3D Genome Sequencing Data

| PRJNA481135 | ENA

Nucleosome mapping of Tetrahymena thermophila chromatin in vivo and in vitro

Project description:A conserved hallmark of eukaryotic chromatin architecture is the distinctive array of well-positioned nucleosomes downstream of transcription start sites (TSS). Recent studies indicate that trans-acting factors establish this stereotypical array. Here, we present the first genome-wide in vitro and in vivo nucleosome maps for the ciliate Tetrahymena thermophila. In contrast with previous studies in yeast, we find that the stereotypical nucleosome array is preserved in the in vitro reconstituted map, which is governed only by the DNA sequence preferences of nucleosomes. Remarkably, this average in vitro pattern arises from subsets of nucleosomes, rather than the whole array, being present in individual Tetrahymena genes. Variation in GC content contributes to the positioning of these sequence-directed nucleosomes, and affects codon usage and amino acid composition in genes. We propose that these ‘seed’ nucleosomes may aid the AT-rich Tetrahymena genome – which is intrinsically unfavorable for nucleosome formation – in establishing nucleosome arrays in vivo in concert with trans-acting factors, while minimizing changes to the coding sequences they are embedded within. All data are from the macronuclear genome. Datasets: 1) Log-phase cells, fixed chromatin, light MNase digest; 2) Log-phase cells, native chromatin, heavy MNase digest; 3) Starved cells, fixed chromatin, light MNase digest; 4) Starved cells, native chromatin, heavy MNase digest; 5) in vitro reconstituted chromatin, 50ul reaction, 4:10 histone:DNA ratio, light MNase digest; 6) in vitro reconstituted chromatin, 50ul reaction, 7:10 histone:DNA ratio, light MNase digest; 7) in vitro reconstituted chromatin, 150ul reaction, 4:10 histone:DNA ratio, light MNase digest; 8) in vitro reconstituted chromatin, 150ul reaction, 4:10 histone:DNA ratio, heavy MNase digest; Control dataset: 9): MNase-digested naked DNA

2015-07-30 | E-GEOD-64061 | biostudies-arrayexpress

Tetrahymena thermophila

Project description:Sequence, Biogenesis, and Function of Diverse sRNA Classes Bound to the Piwi-family Proteins of Tetrahymena thermophila

| PRJNA117745 | ENA