Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Homo sapiens

ABSTRACT: Next Generation Sequencing Analysis of control and DEHP exposure HepG2 cell Transcriptomes

PROVIDER: PRJNA325590 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR3664031.fastq.gz	Fastqsanger.gz
	SRR3664032.fastq.gz	Fastqsanger.gz

Items per page:

1 - 2 of 2

Similar Datasets

Next Generation Sequencing Analysis of control and DEHP exposure HepG2 cell Transcriptomes

Project description:we treated the HepG2 cells with low concentration or control solvent for a long time. Then we extracted the RNAs and performed the next generation sequencing. By comparing sequcing data from control and DEHP treated samples, we profiled the gene expression regulated by low concentration exposure.

2016-08-30 | GSE83321 | GEO

Prenatal and ancestral exposure to di(2-ethylhexyl) phthalate alters gene expression and DNA methylation in mouse ovaries.

Project description:Di(2-ethylhexyl) phthalate (DEHP) is a commonly used plasticizer and known endocrine disrupting chemical, which causes transgenerational reproductive toxicity in female rodents. However, the mechanisms of action underlying the transgenerational toxicity of DEHP are not understood. Therefore, this study determined the effects of prenatal and ancestral DEHP exposure on various ovarian pathways in the F1, F2, and F3 generations of mice. Pregnant CD-1 dams were orally exposed to corn oil (vehicle control) or DEHP (20 μg/kg/day-750 mg/kg/day) from gestation day 10.5 until birth. At postnatal day 21 for all generations, ovaries were removed for gene expression analysis of various ovarian pathways and for 5-methyl cytosine (5-mC) quantification. In the F1 generation, prenatal DEHP exposure disrupted the expression of cell cycle regulators, the expression of peroxisome-proliferator activating receptors, and the percentage of 5-mC compared to control. In the F2 generation, exposure to DEHP decreased the expression of steroidogenic enzymes, apoptosis factors, and ten-eleven translocation compared to controls. It also dysregulated the expression of phosphoinositide 3-kinase (PI3K) factors. In the F3 generation, ancestral DEHP exposure decreased the expression of steroidogenic enzymes, PI3K factors, cell cycle regulators, apoptosis factors, Esr2, DNA methylation mediators, and the percentage of 5-mC compared to controls. Overall, the data show that prenatal and ancestral DEHP exposure greatly suppress gene expression of pathways required for folliculogenesis and steroidogenesis in the ovary in a transgenerational manner and that gene expression may be influenced by DNA methylation. These results provide insight into some of the mechanisms of DEHP-mediated toxicity in the ovary across generations.

2020-09-22 | GSE158266 | GEO

Homo sapiens

Project description:Next Generation Sequencing Analysis of control and siSRSF1 treated RKO cell Transcriptomes

| PRJNA385313 | ENA

Homo sapiens

Project description:Next Generation Sequencing Analysis of control and SRSF2-/- Huh7 cells Transcriptomes

| PRJNA313267 | ENA

Homo sapiens

Project description:Next Generation Sequencing Analysis of control and hnRNPK-/- RKO cells Transcriptomes

| PRJNA338737 | ENA

Homo sapiens

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of KMT2D Knockdown/Control PC-3 Cell Line Transcriptomes

| PRJNA374489 | ENA

Homo sapiens

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of IFNα Stimulated Transcriptomes in HepG2, SETD2-KO, STAT1-KO and STAT1-K525ARe HepG2 cells

| PRJNA384926 | ENA

Next Generation Sequencing Analysis of Control and SETDB1-KO H1299 cell Transcriptomes

Project description:Next Generation Sequencing Analysis of Control and SETDB1-KO H1299 cell Transcriptomes

| PRJNA822618 | ENA

Next Generation Sequencing Analysis of control and siCHERP treated RKO cell Transcriptomes

Project description:Next Generation Sequencing Analysis of control and siCHERP treated RKO cell Transcriptomes

| PRJNA474532 | ENA

Homo sapiens

Project description:Next Generation Sequencing Analysis of ADHFE1 and vector control Transcriptomes in breast cancer cells

| PRJNA319220 | ENA