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A Unique Twist-Dependent Progenitor Cell Contributes to Adult Skeletal Muscle

ABSTRACT: A Unique Twist-Dependent Progenitor Cell Contributes to Adult Skeletal Muscle

PROVIDER: PRJNA329005 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3901910_1.fastq.gz	Fastqsanger.gz
	SRR3901910_2.fastq.gz	Fastqsanger.gz
	SRR3902080_1.fastq.gz	Fastqsanger.gz
	SRR3902080_2.fastq.gz	Fastqsanger.gz
	SRR3902090_1.fastq.gz	Fastqsanger.gz

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A Unique Twist-Dependent Progenitor Cell Contributes to Adult Skeletal Muscle [Tw2 overexpression]

Project description:Skeletal muscle possesses remarkable regenerative potential due to satellite cells, a stem cell population located beneath the muscle basal lamina. By lineage tracing of progenitor cells expressing the Twist2 (Tw2) transcription factor in mice, we discovered a unique myogenic lineage that resides outside the basal lamina of adult muscle and contributes specifically to type IIb/x myofibers during adulthood and muscle regeneration. Tw2+ progenitors are molecularly and anatomically distinct from satellite cells, are highly myogenic in vitro and can fuse with satellite cells. Transplantation of Tw2+ progenitors into adult mice is sufficient to reconstitute new myofibers, and genetic ablation of endogenous Tw2+ progenitors causes wasting of type IIb myofibers. We show that Tw2 expression maintains progenitor cells in an undifferentiated state that is poised to initiate myogenesis in response to appropriate cues that suppress Tw2 expression. Tw2-expressing progenitors represent a previously unrecognized, fiber-type specific progenitor cell involved in muscle growth and regeneration.

2017-01-13 | GSE84377 | GEO

A Unique Twist-Dependent Progenitor Cell Contributes to Adult Skeletal Muscle [Tw2 vs Pax7]

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A Unique Twist-Dependent Progenitor Cell Contributes to Adult Skeletal Muscle [Tw2 vs Pax7 fresh sort]

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A Unique Twist-Dependent Progenitor Cell Contributes to Adult Skeletal Muscle

Project description:This SuperSeries is composed of the SubSeries listed below.

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Project description:A Unique Twist-Dependent Progenitor Cell Contributes to Adult Skeletal Muscle [Tw2 overexpression]

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Project description:A Unique Twist-Dependent Progenitor Cell Contributes to Adult Skeletal Muscle [Tw2 vs Pax7]

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Project description:A Unique Twist-Dependent Progenitor Cell Contributes to Adult Skeletal Muscle [Tw2 vs Pax7 fresh sort]

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ChIP-chip from DmD8 cells with Twist antibodies after 30 minutes Notch activation

Project description:The transcription factor Twist is a critical cooperating factor that confers transcriptional specificity to the Notch pathway in muscle progenitor cells (DmD8) ChIP analysis of Twi show that Twist binding is significantly enriched in Notch responsive region in DmD8 cells

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ChIP-chip from DmD8 cells with Twist antibodies after 30 minutes Notch activation

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Label-free quantification (LFQ) analysis on skeletal muscle stem cells and reprogrammed skeletal muscle stem and progenitor cells

Project description:Direct lineage reprogramming provides a unique system to study cell fate transitions and unearth molecular mechanisms that safeguard cellular identity. We previously reported on direct conversion of mouse fibroblasts into induced myogenic progenitor cells (iMPCs) by transient MyoD overexpression in concert with small molecules treatment. Here we employed integrative multi-omic approaches to delineate the molecular landscape of fibroblast reprogramming into iMPCs in comparison to transdifferentiation into myogenic cells solely by MyoD overexpression. Utilizing bulk RNA-sequencing and mass spectrometry, we uncovered molecular regulators and pathways that endow a myogenic stem cell identity on fibroblasts only in the presence of small molecule treatment. In addition, we demonstrate that Pax7+ cells in iMPCs share molecular attributes with myoblasts, however in addition express unique genes, proteins and pathways that are indicative of a more activated satellite cell-like state in vitro. Collectively, this study charts a molecular blueprint for reprogramming fibroblasts into muscle stem and progenitor cells and further establishes the fidelity of stable iMPC cultures in capturing skeletal muscle regeneration in vitro for disease modeling and basic research applications.

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