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Ambystoma mexicanum strain:AGSC Lab Axolotl

ABSTRACT: Ambystoma mexicanum

PROVIDER: PRJNA33423 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR029144.fastq.gz	Fastqsanger.gz
	SRR029145.fastq.gz	Fastqsanger.gz
	SRR029146.fastq.gz	Fastqsanger.gz
	SRR029147.fastq.gz	Fastqsanger.gz
	SRR029148.fastq.gz	Fastqsanger.gz

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Project description:Asymmetrical localization of biomolecules inside the egg, results in uneven cell division and two daughter cells with different fates. This phenomenon is required for the establishment of many biological processes and is particularly responsible for the great variety of cell types formed during developmentand requires strict timing and positional control. The key molecules determining the body plan are the mRNAs, of which many examples have already been discovered to be asymmetrically localized during oogenesis and embryogenesis in both the amphibian and fish models. However, our knowledge about evolutionary conservation or differences of localized mRNAs is still limited to a few candidates. Our goal has been to compare localization profiles along the animal-vegetal axis of mature eggs of four diverse models, Xenopus laevis, Danio rerio, Ambystoma mexicanum and Acipenser ruthenus using the spatial expression analysis method called TOMO-Seq. Surprisingly, we revealed RNAs that code for many known important genes such as germ layer determinants, germ plasm factors and members of key signalling pathways, are localized in completely different profiles among the models and sometimes even missing in their genomes. We determined the transcriptome distribution and found a poor correlation between the vegetally localized genes but a relatively good correlation between the animally localized genes. These findings indicate that the regulation of embryonic development within the animal kingdom is highly diverse and cannot be deduced based on a single model.

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