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Mus musculus

ABSTRACT: SALL4 ensures oocytes maturation by modulating DNA methylation and histone modifications (RNA-Seq)

PROVIDER: PRJNA335647 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3957130_1.fastq.gz	Fastqsanger.gz
	SRR3957130_2.fastq.gz	Fastqsanger.gz
	SRR3957140_1.fastq.gz	Fastqsanger.gz
	SRR3957140_2.fastq.gz	Fastqsanger.gz
	SRR3957131_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

Mus musculus

Project description:SALL4 ensures oocytes maturation by modulating DNA methylation and histone modifications (RRBS)

| PRJNA335648 | ENA

SALL4 ensures oocytes maturation by modulating DNA methylation and histone modifications

Project description:SALL4 ensures oocytes maturation by modulating DNA methylation and histone modifications

| PRJNA335644 | ENA

SALL4 ensures oocytes maturation by modulating DNA methylation and histone modifications (RRBS)

Project description:Splat-like 4 (Sall4) plays important roles in maintaining pluripotency of embryonic stem cells and in various developmental processes. Here, we find that the SALL4 null oocytes fail to undergo maturation to form fully-grown oocytes (FGOs) and subsequent meiosis resumption. We further discover that the loss of maternal SALL4 causes failure in establishment of DNA methylation. Moreover, we demonstrate that SALL4 modulates H3K4me3 and H3K27me3 modifications by regulating the expression of Kdm5b, Kdm6a and Kdm6b. Taken together, SALL4 plays pivotal roles in oocyte epigenetic maturation.

2017-03-07 | GSE84923 | GEO

SALL4 ensures oocytes maturation by modulating DNA methylation and histone modifications (RNA-Seq)

2017-03-07 | GSE84922 | GEO

Mus musculus

Project description:Dynamic Changes in Histone Modifications Precede de novo DNA Methylation in Oocytes

| PRJNA300683 | ENA

Dynamic Changes in Histone Modifications Precede de novo DNA Methylation in Oocytes

Project description:Erasure and subsequent re-instatement of DNA methylation in the germline, especially at imprinted CpG islands (CGIs), is crucial to embryogenesis in mammals. The mechanisms underlying DNA methylation establishment remain poorly understood, but a number of post-translational modifications of histones are implicated in antagonizing or recruiting the de novo DNA methylation complex. In mouse oogenesis, DNA methylation establishment occurs on a largely unmethylated genome and in non-dividing cells, making it a highly informative model for examining how histone modifications can shape the DNA methylome. Using a chromatin immunoprecipitation and genome-wide sequencing (ChIP-Seq) protocol optimized for low cell numbers and novel techniques for isolating primary and growing oocytes, profiles were generated for histone modifications implicated in promoting or inhibiting DNA methylation. CGIs destined for DNA methylation show reduced protective H3K4me2 and H3K4me3 in both primary and growing oocytes, while permissive H3K36me3 increases specifically at these CGIs in growing oocytes. Methylome profiling of oocytes deficient in H3K4 demethylases KDM1A or KDM1B indicated that removal of H3K4 methylation is necessary for proper methylation establishment at CGIs. This work represents the first systematic study performing ChIP-Seq in oocytes, and shows that histone remodeling in the mammalian oocyte helps direct de novo DNA methylation events.

2015-12-02 | GSE74549 | GEO

Mus musculus musculus

Project description:H3K4 methylation orchestrates DNA methylation in oocytes

| PRJNA906481 | ENA

CFP1 Regulates Histone H3K4 Trimethylation and Developmental Potential in Mouse Oocytes

Project description:Through RNA-seq of wildtype and CFP1-deleted GV oocytes of different ages, zygotes and 2-cell embryos and whole genome bisulfite sequencing of GV oocytes, We show here that CFP1 is responsible for epigenetic maturation in oocytes. Deletion of CFP1 directly decreased histone H3K4 trimethylation and caused global down-regulation of gene expression in oocytes.These genes are involved in cytoplasmic lattice formation, maternal-zygotic transition and epigenetic maturation. Maternal CFP1-deleted oocytes had fewer CPLs in the cytoplasm and the organelles were severely aggregated, which further caused defects in α-tubulin polymerization and aneuploidy in meiosis II. The genome was less methylated and methylation of maternal DNA was impaired after CFP1 deletion. Therefore CFP1-deleted oocytes fail to complete epigenetic maturation as well as cytoplasmic maturation and nuclear maturation and unable to gain developmental competence during oogenesis.

2017-09-30 | GSE85019 | GEO

Finding missing proteins from the epigenetically manipulated human cell with stringent quality criteria

Project description:There are three major epigenetic mechanisms, DNA methylation, histone modifications, and ncRNAs. The histone is a key player in epigenetics, and the acetylation and methylation are their most common post-translational modifications (PTMs). These histone modifications have important roles in transcriptional regulation, DNA repair, DNA replication, alternative splicing and chromosome condensation. For example, we previously found that H3.3 lysine 36 trimethylation (H3.3K36me3) histone and its reader protein BS69 could work together to regulate pre-mRNA process. Therefore, in this study, we established in vitro histone acetylation, demethylation and methylation models, respectively, by using human lung, liver and colorectal cancer cells.

2022-03-02 | PXD002453 | Pride

Maintenance of DNA methylation in embryonic stem cells depends on the histone H3K9 methyltransferases (Dnmt3ab KO ChIP-Seq)

Project description:During mammalian development DNA methylation patterns need to be reset in primordial germ cells (PGC) and preimplantation embryos. However, many retro-transposons and imprinted genes are resistant to such global epigenetic reprogramming via hitherto undefined mechanisms. Here, we report that some of these sequences are immune to widespread erasure of DNA methylation in the mouse embryonic stem cells (mESCs) lacking de novo DNA methyltransferases. Persistence of DNA methylation at these loci in mESCs depends on the histone H3K9 methyltransferase Setdb1, as deletion of Setdb1 results in reduction of H3K9me3 and DNA methylation levels concomitant with an increase in 5-hydroxymethylation (5hmC). In addition, depletion of H3K9 methyltransferase G9a leads to genome-wide reduction of DNA methylation but to a lesser extent at the above sequences. Taken together, these data reveal that Setdb1 ensures the fidelity of DNA methylation at specific loci in mESCs, which may reflect mechanisms functioning in vivo during key developmental stages. Examination of H3K9me3 histone modifications in 2 cell types.

2014-06-03 | E-GEOD-47878 | biostudies-arrayexpress

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