Arabidopsis thaliana
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA335848 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Chloroplast-nuclear retrograde signaling is viewed as a mechanism for inter-organelle communication. Here we show the SAL1-PAP (3′-phosphoadenosine 5′- phosphate) retrograde pathway functions more broadly in guard cells, interacting with abscisic acid (ABA) signaling at least in part via exoribonucleases. Unexpectedly, PAP bypasses the canonical signaling components ABA Insensitive 1 (ABI1) and Open Stomata 1 (OST1) by priming an alternative pathway that restores ABA-responsive gene expression, ROS bursts, ion channel function and stomatal closure in ost1-2. This alternative pathway up-regulates lowly expressed Calcium Dependent Protein Kinases (CDPKs) which have the capacity to activate the key slow anion channel SLAC1 in response to ABA-mediated and ost1-2 independent calcium release. The role of PAP in priming an alternative pathway to bypass components previously considered essential for stomatal closure demonstrates how a chloroplast signal can have broader roles as a secondary messenger to directly intersect with and tune hormone signaling.
2017-03-21 | GSE84997 | GEO
Project description:Transcriptome profiling of WT, mbd1, pyl5 and mbd1 pyl5 knock-out mutants in response to Abscisic acid (ABA) was done using Affymetrix GenechipTM Arabidopsis ATH1 Genome Array.
2024-11-01 | GSE271692 | GEO
Project description:flg22 treatment & mutants-Gene profiling upon biotic stress treatment. Gene profiling in microRNA mutants.
| PRJNA108123 | ENA
Project description:To identify genes of the guard cell transkriptome of Arabidopsis thaliana enriched guard cell samples were compared with total leaf tissue. Genes of the abscisic acid and humidity response of Arabidopsis thaliana guard cells were identified by treatment with ABA-Spray and low humidity. Ost1-2 and slac1-3 mutants were compared to their wildtype.
2013-06-04 | GSE38639 | GEO
Project description:flg22 treatment & mutants 2-Gene profiling upon biotic stress treatment. Gene profiling in microRNA mutants.
| PRJNA105609 | ENA
Project description:Stresses that target mitochondrial function lead to altered transcriptional responses for 100-1000s of genes genome wide, and are signalled via retrograde signalling pathways within the cell. rao1 mutants contain a mutation in a gene encoding a Cyclin-Dependant Kinase E;1 and cannot induce stress responsive genes (such as the mitochondrial alternative oxidase 1a) in response to mitochondrial dysfunction. We sought to define the global gene network regulated through RAO1 function in response to mitochondrial stress (mimicked through treatment of plants with antimycin A - a specific inhibitor of complex III in the mitochondrial electron transfer chain). We have defined global stress responses that are positively and negatively mediated by RAO1 function, as well as global stress responses to antimycin A treatment that are regulated independently of RAO1. We used Affymetrix microarray to characterise global gene expression profiles for mutant plants with compromised mitochondrial retrograde signalling under stress (rao1 mutants), to define the genome wide transcriptional network regulated through RAO1 function. rao1 and wild type 14-day-old seedlings (the optimal stage as defined by forward genetic screens that identifed rao1 mutants) grown on GamborgB5 plates were treated by spraying plants with 50 µM antimycin A (an elicitor of mitochondrial retrograde signalling) while mock control samples were sprayed with deionised water. Samples were collected after 3hr of treatment for global expression profiling.
2013-01-28 | E-GEOD-36011 | biostudies-arrayexpress
Project description:abaseed-ABA signaling in Arabidopsis seeds
| PRJNA281754 | ENA
Project description:translation for mettl5 after ABA treatment
| PRJNA1226565 | ENA
Project description:The role of the highly histone variant H2A.Z remains engimatic, in part because of its context-dependent effects on modulating transcription. Here, we investigate the role of the H2A.Z N-terminal tail in regulating transcriptional responses to the stress hormone abscisic acid (ABA) in Arabidopsis thaliana. In WT plants, treatment with ABA induces hundreds of transcripts to be rapidly up- and downregulated. Using h2a.z null mutant plants expressing either an H2A.Z missing the entire N-temrinal tail (Delta28), or carrying lysine to arginine mutations in either a single lysine (K>R 3) or all nine N-terminal lysines (K>R 1-9), we find that the H2A.Z N-terminus is surprisingly dispensable for the intiation of the ABA response program, but plays an important role in fine-tuning the magnitude of response. Interestingly, distinct subsets of genes exhibit altered response magnitude in each mutant, with limited overlap between genotypes. The most significantly dysregulated of these in the mutants compared to WT are associated with chromatin context: genes upregulated by ABA are enriched for gene-body H2A.Z (gbH2A.Z) at baseline, and show increased expression in mutants primarily under mock (non-ABA) conditions. Meanwhile, ABA-repressed genes tend to be enriched for transcription start site (TSS)-localized H2A.Z at baseline, and exhibit enhanced repression in mutants upon ABA treatment. Notably, the K>R 1-9 mutant produces more differentially expressed genes (DEGs) compared to WT in both mock and ABA-treated conditions that does complete removal of the tail, indiciating that loss of modifiability is not equivalent to loss of the N-terminus. Together, our results support a model in which the major regulatory functions of H2A.Z reside in the highly conserved histone fold domain, while the more evolutionarily variable N-terminal tail acts as a bidirectional buffer to fine-tune transcirptional output. While dispensable for the broad execution of transcriptional programs, this fine-tuning capacity is critical for maintaining transcriptional precision during stress responses, with implications for organismal fitness.
2026-07-24 | GSE338700 | GEO