Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Drosophila melanogaster

ABSTRACT: Transcripts enriched in microsome fraction in ribosome-independent manner

PROVIDER: PRJNA339298 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR4040080.fastq.gz	Fastqsanger.gz
	SRR4040077.fastq.gz	Fastqsanger.gz
	SRR4040078.fastq.gz	Fastqsanger.gz
	SRR4040079.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Transcripts enriched in microsome fraction in ribosome-independent manner

Project description:To identify the transcripts fractionated into microsome fraction in ribosome-independent manner, we isolate rough microsome fraction by sucrose density gradient ultracengrifugation, then the rough microsome fraction is centrifugated following treatment with puromycine and EDTA in high-salt buffer to remove ribosomes. The pellet and surpernatant are named naked microsome fraction (NM) and stripped ribosome fraction (SR), respectively. By calculating the ratio of the level of each mRNA in NM and SR, we identify the enriched transcripts in NM.

2016-12-25 | GSE85768 | GEO

Gel-free mass spectrometry analysis of Drosophila melanogaster heads

Project description:Here we describe a bottom-up approach to analyse an enriched membrane fraction from Drosophila melanogaster heads using multidimensional liquid chromatography (LC) coupled with tandem-mass spectrometry (MS/MS) that relies on a complete solubilisation and digestion of proteins. An enriched membrane fraction was prepared using equilibrium density centrifugation on a discontinual sucrose gradient, followed by solubilisation using FASP method, tryptic and consequential chymotryptic digestion of proteins. Peptides were separated by reversed-phase (RP) LC at high pH in the first dimension and acidic RP-LC coupled directly to Orbitrap Velos Pro mass spectrometer.

2015-07-27 | PXD001712 | Pride

Drosophila melanogaster

Project description:Ribosome footprinting of fly heads

| PRJNA316472 | ENA

Drosophila melanogaster

Project description:Detecting novel low-abundant transcripts in Drosophila

| PRJNA91637 | ENA

Drosophila melanogaster

Project description:Expression profiling of HNF4-dependent transcripts in Drosophila larvae

| PRJNA111609 | ENA

Drosophila melanogaster

Project description:Expression profiling of DHR38-dependent transcripts in Drosophila larvae

| PRJNA131153 | ENA

Drosophila melanogaster

Project description:Transcriptional and ribosome profiling of Drosophila 3rd instar larvae muscle

| PRJNA390134 | ENA

Drosophila melanogaster

Project description:Cell-type-specific gene expression profiling using ribosome affinity purification

| PRJNA354320 | ENA

Ribosome profiling reveals pervasive and regulated stop codon readthrough in Drosophila melanogaster

Project description:Ribosomes can read through stop codons in a regulated manner, elongating rather than terminating the nascent peptide. Stop codon readthrough is essential to diverse viruses, and phylogenetically predicted to occur in a few hundred genes in Drosophila melanogaster, but the importance of regulated readthrough in eukaryotes remains largely unexplored. Here, we present a ribosome profiling assay (deep sequencing of ribosome-protected mRNA fragments) for Drosophila melanogaster, and provide the first genome-wide experimental analysis of readthrough. Readthrough is far more pervasive than expected: the vast majority of readthrough events evolved within D. melanogaster and were not predicted phylogenetically. The resulting C-terminal protein extensions show evidence of selection, contain functional subcellular localization signals, and their readthrough is regulated, arguing for their importance. We further demonstrate that readthrough occurs in yeast and humans. Readthrough thus provides general mechanisms both to regulate gene expression and function, and to add plasticity to the proteome during evolution. 12 samples of Drosophila ribosome profiling and poly(A)+ mRNA-seq, including technical replicates in S2 cells, and biological replicates of 0-2 hour embryos

2013-12-05 | E-GEOD-49197 | biostudies-arrayexpress

Drosophila melanogaster

Project description:Expression data from the mushroom bodies of Drosophila-PKA activity dependent manner

| PRJNA139535 | ENA