Project description:Proteomics analysis in Escherichia coli K12 (E. coli K12) at DMP concentrations of 0 mg·kg-1 (CK) and 80 mg·kg-1 (DMP) revealed the toxicity of DMP
Project description:LrhA, YafC, YdcI, and YhaJ are LysR-type transcription regulator in Escherichia coli K12. In this study the transcriptomes of E. coli K12 strain U4 (MG1655 derivative, rph+ flhDC(IS1) dgcJ∷IS1 crl::IS1 ilvG+ ΔlacZ; Yilmaz et al. 2020, J. Bacteriol 203 e00427-20, https://doi.org/10.1128/JB.00427-20) and isogenic ΔlrhA, ΔyafC, ΔydcI, and ΔyhaJ mutants grown in Tryptone medium to OD600 0.8 were determined. The data revealed few loci that may be specifically affected in one deletion mutant only, while most differentially expressed loci were up or down regulated in several of the mutants.
Project description:We evaluated both the transcriptomic and inflammatory response in trout (O. mykiss) macrophages in primary cell culture stimulated with DAP-PGN (DAP; meso-diaminopimelic acid, PGN; peptidoglycan) from two strains of Escherichia coli (PGN-K12 and PGN-O111:B4) over time. Transcript profiling was assessed using function-targeted cDNA microarrays hibridation (n = 36) to differential responses to both PGNs that are both time and treatment dependen over trout macrophages. Wild type E. coli (K12) generated an increase in transcript number/diversity over time whereas PGN-O111:B4 stimulation resulted in a more specific and intense response. In line with this gene Ontology analysis (GO) highlights a specific transcriptomic remodelling for PGN-O111:B4 whereas results obtained for PGN-K12 shows a high similarity with a general LPS response where multiple functional classes are related to ribosome biogenesis or cellular metabolism
