Project description:The C. elegans lifespan in the presence of Bacillus licheniformis caused induction of a large number of genes associated with anti-aging activiy including beta-oxidation Inaddition, these results indicate the B. licheniformis enhances the lifespan of Caenorhabditis elegans through serotonin signaling
Project description:The C. elegans lifespan in the presence of Bacillus licheniformis caused induction of a large number of genes associated with anti-aging activiy including beta-oxidation Inaddition, these results indicate the B. licheniformis enhances the lifespan of Caenorhabditis elegans through serotonin signaling Two-condition experiment, C. elegans with B. lichemiformis 141 or E. coli OP50 (conrol) for 24 h. For preparing the total RNA, C. elegans were exposed to 20 mg of bacterial lawn in NGN agar for 24 h.
Project description:Proteomes of Bacillus licheniformis DSM13 cultivated with glucose, rhamnose as well as ulvan or two ulvan hydrolysates (UHA and UHB). Hydrolysates were generated using selected Formosa agariphila KM3901 ulvan-PUL encoded enzymes.
Project description:<p>This study investigated the effects of Bacillus licheniformis supplementation on the gut metabolic profile of patients with mild-to-moderate active ulcerative colitis (UC). Patients received either mesalazine alone or mesalazine combined with Bacillus licheniformis. Fecal samples were collected and subjected to untargeted LC-MS/MS metabolomics analysis. Bacillus licheniformis intervention was associated with alterations in the gut microbiota and metabolite profiles, including enrichment of Prevotella and increased levels of nicotinamide (NAM). Multi-omics analysis indicated a close relationship between Prevotella abundance and NAM metabolism. Further experimental validation suggested that Bacillus licheniformis interacts with Prevotella through bacterial cross-feeding, contributing to increased NAM production and activation of the Wnt signaling pathway. These findings provide evidence for a potential Bacillus licheniformis–Prevotella–nicotinamide–Wnt axis involved in the alleviation of ulcerative colitis.</p>