Project description:We used Affymetrix microarray to survey the global gene expression changes in adipocytes that have been treated with acute lymphoblastic leukemia cell conditioned media.
Project description:miRNA profiles of adipocyte-derived microvesicles (ADMs) on the Day 2-4 and Day 8-10 were compared. ADMs were prepared from the 48h-conditioned medium of 3T3-L1 adipocytes (Day 2-4 and Day 8-10) followed by RNA isolation.
Project description:This SuperSeries is composed of the following subset Series: GSE18598: Differentiating 3T3-L1 adipocytes, introduced with siRNA against aof2 and rfk genes, or treated with tranylcypromine GSE18599: Differentiating 3T3-L1 adipocytes, introduced with siRNA against phf21a gene Refer to individual Series
Project description:3T3-L1 pre-adipocytes were differentiated into mature adipocytes over 10 days using MDI induction and insulin supplementation. Differentiated adipocytes were treated with 12.5 nM citrate-capped gold nanoparticles for 24 hours. Total RNA was extracted and analyzed using the Mouse Adipogenesis RT² Profiler PCR Array (Qiagen) to quantify gene expression changes in adipogenesis, lipid metabolism, and related pathways. Untreated differentiated 3T3-L1 cells served as controls.
