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Homo sapiens

ABSTRACT: Gene expression of collecting duct carcinoma of the kidney

PROVIDER: PRJNA350320 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR4448020_1.fastq.gz	Fastqsanger.gz
	SRR4448020_2.fastq.gz	Fastqsanger.gz
	SRR4448021_1.fastq.gz	Fastqsanger.gz
	SRR4448021_2.fastq.gz	Fastqsanger.gz
	SRR4448022_1.fastq.gz	Fastqsanger.gz

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Project description:Transcriptional profiling of new born mouse kidney collecting duct (CD) cells comparing the infuence of gestational high salt stress on gene expression remolding of BdkrB2 receptor knockout CD cells with that of BdkrB2 receptor wild type CD cells. The BdkrB2 receptor has been shown to be playing a role in renal vascular tone, kidney secretion and reabsorption function, normal kidney development, while impaired BdkrB2 receptor in kidney shown being associated with renal agenesis and renal dysplasia. Goal was to determine the effects of BdkrB2 receptor knockout together with gestational high salt stress on collecting duct gene expression pattern.

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Project description:miRNA plays a role as post-transcriptional regulator. However, miRNAs in the kidney collecting duct cell have not been well understood. So we aimed to profile miRNAs in the kidney inner medullary collecting duct (IMCD) cells, and to identify the vasopressin-responsive miRNAs in the kidney IMCD cells. The microarray assay revealed that relative expression of miRNAs in the kidney IMCD cells was changed by desmopressin (dDAVP) stimulation. Fresh IMCD tubules were prepared from rat kidney and incubated in the medium in the absence or presence of dDAVP (1 nM, 2 h). Total RNA purified from IMCD tubules was used to analyze miRNA expression by GeneChipM-BM-. miRNA 3.0 Array (Affymetrix).

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Project description:This experiment aimed to uncover the genome-wide alternation in gene expression resulting from the removal of PRR from the population ureteric bud and collecting duct and successive changes to the series of events in kidney development.

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